PMID: 7541828Jul 15, 1995Paper

T cell recognition of immunodominant and cryptic proteolipid protein epitopes in humans

The Journal of Immunology : Official Journal of the American Association of Immunologists
S Markovic-PleseD A Hafler

Abstract

We investigated the immune response to proteolipid protein (PLP), the most abundant central nervous system myelin protein in humans. A total of 8207 short-term T cell lines were generated from 49 individuals, 39 patients with multiple sclerosis and 10 control subjects. As we have reported previously, the frequency of PLP-reactive T cells did not differ between the two groups. To determine immunodominant PLP epitopes, proliferative responses of 971 PLP-specific lines were tested with 27 overlapping 20-amino acid peptides encompassing the human PLP sequence and the binding affinities of the PLP peptides to DRB5*0101 and DRB1*1501, DR2 MHC class II isotypes associated with multiple sclerosis, were determined. The T cell response after primary PLP stimulation was focused on two immunodominant epitopes comprising residues p30-49 and p180-199. These two fragments were recognized after processing of native protein by APCs and were situated in hydrophilic regions of PLP exhibiting only moderate affinity to DR2 molecules. In contrast, when T cells from DR2+ subjects were stimulated initially by individual synthetic peptides with either high or low affinity to DRB5*0101 and DRB1*1501 isotypes, additional cryptic epitopes were recognized....Continue Reading

Citations

Dec 14, 2002·Journal of the Neurological Sciences·Permphan Dharmasaroja
May 14, 2003·Journal of Neuroimmunology·C Arturo SolaresVincent K Tuohy
Mar 1, 2000·Journal of Neuroimmunology·A Bar-OrD A Hafler
Dec 5, 2002·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Michael P PenderJudith M Greer
Jan 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Joel N H SternJack L Strominger
Apr 1, 1997·The Journal of Clinical Investigation·V K TuohyR P Kinkel
Jun 22, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Annie Elong NgonoDavid-Axel Laplaud
Sep 30, 2017·Iranian Journal of Biotechnology·Zahra ZamanzadehMohammad Hosein Sanati

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