T-cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection.

Immunology
Marcela Helena Gonçalves PereiraHelton da Costa Santiago

Abstract

Multifunctional interleukin 10 (IL10)+ Th1 cells have been implicated in favorable evolution of many infectious diseases, promoting an efficacious immune response while limiting immunopathology. Here, we investigated the presence of multifunctional CD4+ and CD8+ T-cells that expressed interferon gamma (IFNγ), IL10 and tumor necrosis factor (TNF), or its combinations during dengue infection. Peripheral blood mononuclear cells (PBMCs) from outpatients with dengue (mild dengue forms) and hospitalized patients (or patients with dengue with warning signs and severe dengue) were cultured in the presence of envelope (ENV) or NS3 peptide libraries of DENV during critical (hospitalization period) and convalescence phases. The production of IFNγ, IL10 and TNF by CD4+ and CD8+ T-cells was assessed by flow cytometry. Our data show that patients with mild dengue, when compared with patients with dengue with warning signs and severe dengue, presented higher frequencies of multifunctional T-cells like NS3-specific IFNγ/IL10-producing CD4+ T-cells in critical phase and NS3- and ENV-specific CD8+ T-cells producing IFNγ/IL10. In addition, NS3-specific CD8+ T-cells producing high levels of IFNγ/TNF and IFNγ/TNF/IL10 were also observed in the mild...Continue Reading

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Citations

Jul 30, 2020·Journal of Leukocyte Biology·Tertuliano Alves Pereira NetoHelton da Costa Santiago
Feb 12, 2021·GeroScience·Jennifer ConnorsElias K Haddad
Jan 31, 2021·NPJ Vaccines·Wei-Xin ChinJustin Jang Hann Chu
Jun 15, 2021·The Journal of Infectious Diseases·Iracema Luisa Quintino-de-CarvalhoHelton Da Costa Santiago
Nov 3, 2021·Science Immunology·Gisella GuerreraGiovanna Borsellino

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