PMID: 11607370Feb 15, 1993Paper

T-DNA transfer to maize cells: histochemical investigation of beta-glucuronidase activity in maize tissues

Proceedings of the National Academy of Sciences of the United States of America
W H ShenZ Koukolíková-Nicola

Abstract

Agrobacterium tumefaciens is routinely used to engineer desirable genes into dicotyledonous plants. However, the economically important graminaceous plant maize is refractory to tumor induction by inoculation with virulent strains of A. tumefaciens. Currently, the only clearcut evidence for transferred DNA (T-DNA) transport from Agrobacterium to maize comes from agroinfection. To study T-DNA transfer from Agrobacterium to maize cells in a virus-free system, we used here the beta-glucuronidase (GUS; EC 3.2.1.31) gene as a marker. GUS expression was observed with high efficiency on shoots of young maize seedlings after cocultivation with Agrobacterium carrying the GUS gene. Agrobacterium virulence mutants, incapable of transferring T-DNA to dicot tissue, were shown to be deficient in eliciting GUS expression in maize. Hence, expression of the T-DNA-located GUS gene in maize cells is strictly dependent on Agrobacterium-mediated DNA transfer. Histochemical staining of maize shoots revealed GUS expression located mainly in the leaves and the coleoptile.

References

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Citations

Sep 1, 1994·Plant Molecular Biology·I K Vasil
Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Philippe RechAlain Jauneau
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·J EscuderoB Hohn
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Stanton B. Gelvin
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Stanton B Gelvin
Aug 9, 2020·Frontiers in Plant Science·Stephanie LiuB Daniel Pierce

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