PMID: 10594570Dec 14, 1999Paper

T helper frequencies in peripheral blood reflect donor-directed reactivity in the graft after clinical heart transplantation

Clinical and Experimental Immunology
L M VaessenW Weimar

Abstract

We describe the usefulness of a fast (48-h) limiting dilution assay (LDA) for the enumeration of human alloreactive helper T lymphocytes (HTL) in the peripheral blood, in relation to histologically defined rejection grades after heart transplantation. HTL frequencies (HTLf) in pretransplant samples varied from patient to patient, ranging from 106 to 625 HTL/106 peripheral blood mononuclear cells (PBMC). In the first week after heart transplantation (HTx), when immunosuppression was instituted, HTLf were significant lower (range 30-190 HTL/106). The level of HTL in the first week after HTx when rejection grade was 0 or 1A (ISHLT score) was considered to be the baseline frequency. This frequency did not correlate with the number of subsequent rejection episodes. During rejection (grade 3), donor-specific HTLf were increased above their baseline frequencies (P = 0.01). Expressed as percentage of baseline frequencies, HTLf increased significantly during acute rejection (AR) compared with 1-2 weeks before rejection (P = 0.003). The increase was specific, since viral infections did not result in a rise of donor-specific HTL, while also HTLf specific for third party HLA antigens were not elevated during rejection. Monitoring HTLf in p...Continue Reading

References

Aug 1, 1990·Immunology Today·C E SharrockS Man
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Jul 1, 1990·Transplantation·J A ReaderJ R Pepper
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Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·L LoonenW Weimar

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Citations

Jun 5, 2001·The Annals of Thoracic Surgery·F B OeiA J Bogers
Aug 8, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·I Esmé DijkeCarla C Baan
Feb 18, 2010·Expert Review of Clinical Immunology·Paolo Cravedi, Roslyn B Mannon
Dec 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M H HammerJ W Kupiec-Weglinski

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