PMID: 29936Sep 1, 1978Paper

T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody

The Journal of Experimental Medicine
R A WhiteC Milstein

Abstract

W3/25 antibody is the monoclonal product of a hybrid cell resulting from the fusion of a mouse myeloma cell line with spleen cells from a mouse immunized with rat thymocytes. Pure clones have been derived, and segregants free of parental myeloma chains have been isolated. Previous studies have shown that this antibody recognizes a subpopulation of T cells among rat thoracic duct lymphocytes. In the work reported here, three T-cell functions were assayed after separating rat thoracic duct lymphocytes on the fluorescence-activated cell sorter on the basis of labeling with W3/25 antibody. Two of the functional activities appeared to be completely segregated by this procedure. Thus, helper cell activity for an anti-hapten plaque-forming cell response was confined to the labeled population, whereas the allogeneic suppressive effect produced in a parental vector F1 adoptive transfer was mediated by cells in the unlabeled fraction. The third function, graft-versus-host activity, was almost entirely contained within the labeled subpopulation. It is concluded that the antigenic determinant recognized by the monoclonal antibody W3/25 is a differentiation marker for T-cell functional subpopulations.

References

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Citations

Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Milstein
Jan 1, 1995·Acta neurochirurgica·S HolminP Biberfeld
Oct 1, 1987·The Histochemical Journal·F Colbatzky, W Hermanns
Sep 1, 1993·Annals of Hematology·A FranchM Castell
Oct 1, 1982·Cellular Immunology·P Del GuercioP Truffa-Bachi
Jan 1, 1982·Veterinary Immunology and Immunopathology·F KristensenS Lazáry
Oct 1, 1992·Veterinary Immunology and Immunopathology·C J WoolvertonR B Sartor
Aug 1, 2000·Immunology Today·C Milstein
Aug 1, 2000·Immunology Today·L A Herzenberg, S C De Rosa
Apr 1, 1997·Nuclear Medicine and Biology·M RusckowskiD J Hnatowich
Dec 12, 2001·Clinical and Experimental Immunology·L D SpargoG Mayrhofer
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I Gozes, C J Barnstable
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S J ClarkA F Williams
Oct 1, 1980·The Journal of Experimental Medicine·E Fernandez-CruzJ D Feldman
Dec 1, 1982·The Journal of Experimental Medicine·A N Barclay, D W Mason
Jun 1, 1983·The Journal of Experimental Medicine·C W PughH W Steer
Jul 1, 1985·The Journal of Experimental Medicine·W A JefferiesA F Williams
Nov 1, 1985·The Journal of Experimental Medicine·J Green, R Jotte
Jan 1, 1987·The Journal of Experimental Medicine·D J PatersonA F Williams

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