PMID: 6978891Apr 1, 1982Paper

T lymphocyte interaction with immunoglobulin G antibody in systemic lupus erythematosus

The Journal of Clinical Investigation
K OkudairaR C Williams

Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disease with multiple immune disturbances whose mechanisms remain unclear. We examined the interaction of antilymphocyte antibodies with cultured normal T lymphocytes. T cells were prepared by E-rosetting after petri-dish removal of adherent cells and cultured for 2-7 d in the presence of SLE sera or normal human sera. Cultured T cells were washed and sonicated, and the amount of cell-associated IgG was quantitated by radioimmunoassay or enzyme-linked immunoassay (ELISA) methods. T cells cultured with 27 of 39 SLE sera showed marked increments of associated immunoglobulin G (IgG) although this was not observed with sera from mixed connective tissue disease patients containing high titers of ribonucleoprotein antibody or normal donors. The effective factors for IgG association in SLE sera were absorbed with normal peripheral blood lymphocytes or T cells. Anti-T cell IgG cytotoxic activity strongly correlated with T cell IgG association (P less than 0.01). T cell-associated IgG was not removed by stripping of cell membrane IgG from living cells by acid buffer treatment; indirect immunofluorescence of cells fixed after 2-4 d of culture revealed cytoplasmic IgG staining. IgG anti-...Continue Reading

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Citations

Mar 1, 1994·Clinical Rheumatology·C Osman, A J Swaak
Jan 1, 1994·Immunologic Research·M H FosterM P Madaio
Jan 1, 1988·The Journal of Clinical Investigation·S MinotaJ Winfield
Aug 30, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Chau-Ching LiuJoseph M Ahearn
Jan 1, 1984·Immunological Communications·C C Hsu
Jun 1, 1987·Arthritis and Rheumatism·K OkudairaR C Williams
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R H WeisbartR Nishimura
Apr 5, 1997·Annals of the New York Academy of Sciences·M P MadaioL Jarett
Apr 1, 1996·Immunology Today·D Alarcon-SegoviaL Llorente

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