T-lymphocyte invasiveness: control by voltage-gated Na+ channel activity

FEBS Letters
Scott P FraserMustafa B A Djamgoz

Abstract

Whole-cell patch-clamp recordings showed that a sub-population (10%) of Jurkat cells, a model of human T-cells, expressed a functional voltage-gated sodium channel, which was tetrodotoxin (TTX)-resistant. Expression of voltage-gated sodium channel protein was confirmed by western blots. Semi-quantitative PCR analysis revealed that mRNAs for the alpha-subunits of multiple voltage-gated sodium channel subtypes were present but indicated that Na(v)1.5 was the predominant subtype, consistent with the TTX-resistant nature of the recorded currents. Importantly, 10 microM TTX reduced the number of Jurkat cells invading a Matrigel basement membrane by 93.0+/-5.5%. Since similar sodium channels have also been detected in normal human T-lymphocytes, it is concluded that the activity of voltage-gated sodium channels could represent a novel mechanism potentiating the invasive capacity of these cells.

References

Nov 6, 1992·Biochimica Et Biophysica Acta·R GáspárS Damjanovich
May 15, 1989·Biochemical and Biophysical Research Communications·C PieriS Damjanovich
Apr 5, 1996·Science·E C Butcher, L J Picker
Jan 1, 1997·British Journal of Cancer·A MarínJ Bello
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Jun 25, 2003·The Journal of Biological Chemistry·Carl D Bortner, John A Cidlowski
Jan 7, 2004·Nature Reviews. Cancer·Jeffrey W Pollard
Apr 2, 2004·Nature Reviews. Immunology·Robert T Abraham, Arthur Weiss
Apr 13, 2004·Journal of Cell Science·Maria E Mycielska, Mustafa B A Djamgoz
May 4, 2004·Trends in Pharmacological Sciences·K George ChandyMichael D Cahalan

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Citations

Dec 20, 2005·The Journal of Membrane Biology·Karl Kunzelmann
Oct 6, 2006·Pflügers Archiv : European journal of physiology·Albrecht SchwabChristian Stock
Jul 14, 2006·Breast Cancer Research and Treatment·William J BrackenburyMustafa B A Djamgoz
Nov 18, 2006·Cancer Metastasis Reviews·Jamie L FiskeRobert A Sikes
Jan 30, 2008·Nature Clinical Practice. Neurology·Stephen G Waxman
Nov 9, 2005·Prostate Cancer and Prostatic Diseases·J K J DissD S Latchman
Sep 11, 2012·Brain : a Journal of Neurology·Niels EijkelkampJohn N Wood
Oct 23, 2008·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·William J BrackenburyLori L Isom
Mar 18, 2006·The Journal of Physiology·William J Brackenbury, Mustafa B A Djamgoz
Sep 4, 2012·Trends in Cardiovascular Medicine·Ge Gao, Samuel C Dudley
Dec 2, 2009·The International Journal of Biochemistry & Cell Biology·Athina-Myrto ChioniMustafa B A Djamgoz
Jun 30, 2009·Toxicon : Official Journal of the International Society on Toxinology·Tod A LeighfieldJohn S Ramsdell
May 20, 2008·Journal of the Neurological Sciences·Joel A Black, Stephen G Waxman
Feb 7, 2006·The International Journal of Biochemistry & Cell Biology·Gianluca FulgenziMaurizio Taglialatela
Dec 2, 2008·The International Journal of Biochemistry & Cell Biology·Athina-Myrto ChioniMustafa B A Djamgoz
Dec 31, 2008·Glia·Joel A BlackStephen G Waxman
Nov 9, 2007·Traffic·Fiona S CusdinAntony P Jackson
May 15, 2007·Biophysical Journal·Catherine E Morris, Peter F Juranka
Feb 8, 2006·International Journal of Pharmaceutics·Ute SteinfeldHyeck-Hee Lee
Apr 30, 2015·Biochimica Et Biophysica Acta·Pierre BessonSébastien Roger
Jul 25, 2015·Acta Biochimica Et Biophysica Sinica·Canan Cakir AktasA Kevser Piskin
Oct 12, 2011·Experimental Neurology·Joel A Black, Stephen G Waxman
Aug 19, 2015·Frontiers in Pharmacology·Sébastien RogerPierre Besson
Oct 22, 2013·Neuron·Joel A Black, Stephen G Waxman
Sep 21, 2012·Channels·William J Brackenbury
Apr 12, 2015·Annual Review of Immunology·Stefan FeskeEdward Y Skolnik
Jun 6, 2013·Journal of Neuroimmunology·Marijke StevensJan Tytgat
Apr 20, 2010·Journal of Molecular and Cellular Cardiology·Annett SchroeterThomas Zimmer
Aug 28, 2013·Journal of Psychiatric Research·Hubertus HimmerichUlrich Sack
Jan 29, 2013·Experimental Neurology·Bernd C Kieseier, Hans-Peter Hartung
May 23, 2012·The Journal of International Medical Research·S-Q SunP-L Huang
Sep 7, 2012·Multiple Sclerosis : Clinical and Laboratory Research·Joel A BlackStephen G Waxman
Sep 3, 2013·Therapeutic Advances in Neurological Disorders·Stefan Bittner, Sven G Meuth
Sep 22, 2006·Nature Reviews. Neuroscience·Leonard K Kaczmarek

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