PMID: 2497158May 1, 1989Paper

T lymphocytes and monocytes bind to keratinocytes in frozen sections of biopsy specimens of normal skin treated with gamma interferon

Journal of the American Academy of Dermatology
B J Nickoloff, C E Griffiths

Abstract

When 2 mm punch biopsy specimens of normal skin are incubated with gamma interferon (IFN-gamma), the keratinocytes are induced to express intercellular adhesion molecule-1 (ICAM-1). Lymphocytes and monocytes that express lymphocyte function-associated antigen-1 (LFA-1) bind to cultured keratinocytes expressing ICAM-1. We have developed an in vitro adherence assay using frozen sections of normal skin that have been incubated with IFN-gamma, and then overlaid by peripheral blood mononuclear leukocytes. Although peripheral blood mononuclear leukocytes do not bind to the epidermal keratinocytes of untreated skin sections, after exposure of the skin to IFN-gamma the peripheral blood mononuclear leukocytes prominently bind to the epidermal keratinocytes that express ICAM-1. The binding by peripheral blood mononuclear leukocytes is increased approximately twofold by activation with phorbol ester treatment. The temperature dependence and kinetics of this adherence reaction reveal no binding at 8 degrees C, good binding at 24 degrees C, and optimal binding at 37 degrees C, reaching a maximal extent by 60 minutes. The adherence reaction is blocked either by pretreating the peripheral blood mononuclear leukocytes with LFA-1 antibody or th...Continue Reading

References

Oct 1, 1987·The American Journal of Dermatopathology·B J NickoloffE C Butcher

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Citations

Apr 1, 1996·The Journal of Pathology·L H JasparsC J Meijer
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