PMID: 6973606Aug 1, 1981Paper

T lymphocytes specific for immunoglobulin allotype. II. Cloned Igh-1b-specific cytotoxic T cells

The Journal of Experimental Medicine
H R SnodgrassD B Wilson

Abstract

This study describes long-term-cultured lines and clones of cytotoxic T cells (Tc) with specificity for determinants of the Igh-1(b) immunoglobulin allotype. These Tc clones were initiated by repeated stimulation of immune spleen cells from BALB/c mice with an Igh-1(b)-producing myeloma, and then they were maintained in medium supplemented with mitogen-induced growth factors in the absence offurther antigenic stimulation . The lytic potency of these clones was 30-100-fold greater than the primary cultures from which they were derived, and specificity studies showed them to be lytic for Igh-1(b) targets and not for targets expressing Igh-1(a) or Igh-4(b), nor the lipopolysaccharide blasts . Finally, soluble preparations of Ig were tested for their ability to block lysis of labeled Igh-1(b)-expressing targets. The results showed that Igh-1(b) and not other immunoglobulin allotypes or isotypes could block lysis, and that the mechanism of lytic inhibition is due to Igh-1(b)-induced autolysis of the killer cells.

References

Jul 14, 1977·Nature·S Gillis, K A Smith
Aug 1, 1978·European Journal of Immunology·M J BosmaJ L Owen
Mar 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·R Hunter
Apr 1, 1980·The Journal of Experimental Medicine·A L Glasebrook, F W Fitch

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Citations

Jul 30, 1982·Journal of Immunological Methods·W Haas, H Von Boehmer
Mar 1, 1989·Research in Immunology·P Benaroch, G Bordenave
Mar 1, 1989·Research in Immunology·C Leclerc
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S MaekawaZ Ovary
Nov 1, 1981·The Journal of Experimental Medicine·J FormanE S Vitetta
Jul 1, 1982·The Journal of Experimental Medicine·L A Sherman
Jan 1, 1982·Immunological Reviews·Y J Rosenberg
Jul 1, 1984·European Journal of Immunology·R Zamoyska, H Waldmann

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