Abstract
This study describes long-term-cultured lines and clones of cytotoxic T cells (Tc) with specificity for determinants of the Igh-1(b) immunoglobulin allotype. These Tc clones were initiated by repeated stimulation of immune spleen cells from BALB/c mice with an Igh-1(b)-producing myeloma, and then they were maintained in medium supplemented with mitogen-induced growth factors in the absence offurther antigenic stimulation . The lytic potency of these clones was 30-100-fold greater than the primary cultures from which they were derived, and specificity studies showed them to be lytic for Igh-1(b) targets and not for targets expressing Igh-1(a) or Igh-4(b), nor the lipopolysaccharide blasts . Finally, soluble preparations of Ig were tested for their ability to block lysis of labeled Igh-1(b)-expressing targets. The results showed that Igh-1(b) and not other immunoglobulin allotypes or isotypes could block lysis, and that the mechanism of lytic inhibition is due to Igh-1(b)-induced autolysis of the killer cells.
References
Jul 14, 1977·Nature·S Gillis, K A Smith
Sep 10, 1976·Science·D A MorganR Gallo
Aug 1, 1978·European Journal of Immunology·M J BosmaJ L Owen
Mar 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·R Hunter
Oct 1, 1980·The Journal of Experimental Medicine·A K AbbasM I Greene
Jan 1, 1981·Immunological Reviews·H von Boehmer, W Haas
Apr 1, 1980·The Journal of Experimental Medicine·A L Glasebrook, F W Fitch
Aug 1, 1981·The Journal of Experimental Medicine·H R SnodgrassM J Bosma
Citations
Jul 30, 1982·Journal of Immunological Methods·W Haas, H Von Boehmer
Mar 1, 1989·Research in Immunology·P Benaroch, G Bordenave
Mar 1, 1989·Research in Immunology·C Leclerc
May 1, 1995·Research in Immunology·L MajlessiG Bordenave
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S MaekawaZ Ovary
Aug 1, 1981·The Journal of Experimental Medicine·H R SnodgrassM J Bosma
Nov 1, 1981·The Journal of Experimental Medicine·J FormanE S Vitetta
Jul 1, 1982·The Journal of Experimental Medicine·L A Sherman
Jan 1, 1982·Immunological Reviews·Y J Rosenberg
Oct 1, 1991·European Journal of Immunology·K Bartnes, K Hannestad
Jul 1, 1986·European Journal of Immunology·P Vieira, K Rajewsky
Oct 1, 1985·European Journal of Immunology·C HuaA M Schmitt-Verhulst
Jul 1, 1984·European Journal of Immunology·R Zamoyska, H Waldmann