T-SPOT.TB: an in vitro diagnostic assay measuring T-cell reaction to Mycobacterium tuberculosis-specific antigens.

Molecular Diagnosis & Therapy
Antona J Wagstaff, Jean-Pierre Zellweger

Abstract

The overall sensitivity of the RD1 ELISPOT assay (T-SPO.TB), which counts T cells sensitized to specific Mycobacterium tuberculosis peptide sequences, was 81-97% in populations with confirmed tuberculosis, in whom 39-78% had immunosuppressive conditions. In patients with confirmed tuberculosis, the respective RD1 ELISPOT versus tuberculin skin test (TST) sensitivities were 100% versus 89% in adults and 77% versus 35% in children (of whom 39% were HIV-positive). In contrast to that of the TST, the sensitivity of the RD1 ELISPOT assay was not significantly affected by age <36 months, or the immunological or nutritional status of the subjects. Specificity was 100% in two UK-based studies. Isolated false positives have been recorded in patients infected with non-tuberculous M. kansasii. A study investigating latent tuberculosis infection found no significant difference in results between HIV-positive and -negative participants for the RD1 ELISPOT assay, while the TST varied significantly with HIV status. Contact-tracing studies have demonstrated concordance between the RD1 ELISPOT assay and the TST of 65-89%. There is a significant correlation between a positive result and the degree of exposure to the index case for the RD1 ELISPO...Continue Reading

References

May 10, 2002·The European Respiratory Journal·J F BroekmansUNKNOWN World Health Organization, International Union Against Tuberculosis and Lung Disease, and Royal Netherlands Tuberculosis Ass
Dec 6, 2002·The New England Journal of Medicine·Kamran KhanJoshua Graff Zivin
Jan 9, 2003·Journal of Clinical Microbiology·Albert R ZinkAndreas G Nerlich
May 14, 2003·Archives of Internal Medicine·Elizabeth L CorbettChristopher Dye
May 8, 2004·American Journal of Respiratory and Critical Care Medicine·Luca RicheldiAjit Lalvani
Jun 3, 2004·Rheumatic Diseases Clinics of North America·Jeffry Bieber, Arthur Kavanaugh
Nov 30, 2004·The Lancet Infectious Diseases·Madhukar PaiJohn M Colford
Dec 23, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Katalin A WilkinsonAjit Lalvani
Apr 20, 2005·Clinics in Chest Medicine·William J Burman
Apr 20, 2005·Clinics in Chest Medicine·Kristina Feja, Lisa Saiman
Apr 20, 2005·Clinics in Chest Medicine·Kelly E Dooley, Timothy R Sterling
Aug 6, 2005·American Journal of Respiratory and Critical Care Medicine·Homayoun ShamsPeter F Barnes
Sep 1, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T MeierT Regnath

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Oct 26, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fuzhen ZhangYu Pang

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