PMID: 8972390Dec 1, 1996Paper

T-type Ca2+ channel lowers the threshold of spike generation in the newt olfactory receptor cell

The Journal of General Physiology
Fusao KawaiA Kaneko

Abstract

Mechanisms underlying action potential generation in the newt olfactory receptor cell were investigated by using the whole-cell version of the patch-clamp technique. Isolated olfactory cells had a resting membrane potential of -70 +/- 9 mV. Injection of a depolarizing current step triggered action potentials under current clamp condition. The amplitude of the action potential was reduced by lowering external Na+ concentration. After a complete removal of Na+, however, cells still showed action potentials which was abolished either by Ca2+ removal or by an application of Ca2+ channel blocker (Co2+ or Ni2+), indicating an involvement of Ca2+ current in spike generation of newt olfactory receptor cells. Under the voltage clamp condition, depolarization of the cell to -40 mV from the holding voltage of -100 mV induced a fast transient inward current, which consisted of Na+ (INa) and T-type Ca2+ (ICa.T) currents. The amplitude of ICa,T was about one fourth of that of INa. Depolarization to more positive voltages also induced L-type Ca2+ current (ICa,L). ICa,L was as small as a few pA in normal Ringer solution. The activating voltage of ICa,T was approximately 10 mV more negative than that of INa. Under current clamp, action potentia...Continue Reading

Citations

Mar 15, 2001·Neuroscience Research·F Kawai, E Miyachi
Jul 23, 2003·Neuroscience Research·Kenji NarusuyeEi-ichi Miyachi
Apr 14, 2007·Visual Neuroscience·Pratip Mitra, Robert F Miller
Oct 3, 2007·Visual Neuroscience·Pratip Mitra, Robert F Miller
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vikas BhandawatKing-Wai Yau
Oct 12, 2001·Chemical Senses·Y Imanaka, H Takeuchi
Oct 17, 2003·The Journal of Physiology·Francesca FieniAlbertino Bigiani
Jan 10, 2003·The Japanese Journal of Physiology·Kyoko WatanabeTakashi Kurahashi
Jun 8, 2001·Neuron·F KawaiE Miyachi
Apr 12, 2005·Journal of Pharmacological Sciences·Ken-ichi OtsuguroToshiyuki Saito
Jul 8, 2017·American Journal of Physiology. Cell Physiology·Yoshihiko WakazonoSusumu Terakawa
Nov 19, 2004·Journal of Neurophysiology·Akihiro Tomaru, Takashi Kurahashi
Apr 30, 1998·Physiological Reviews·D Schild, D Restrepo
Aug 30, 2017·Cellular and Molecular Neurobiology·Kayo HorioEi-Ichi Miyachi
Oct 27, 2017·PloS One·Chaymae El AlaouiPhilippe Lory
Nov 9, 2018·Frontiers in Behavioral Neuroscience·Hiroki HaradaTomoyuki Kuwaki
Dec 1, 2020·Frontiers in Cellular Neuroscience·Colten K LankfordSheila A Baker

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