T-Type Calcium Channels Are Required to Maintain Viability of Neural Progenitor Cells

Biomolecules & Therapeutics
Ji-Woon KimChan Young Shin

Abstract

T-type calcium channels are low voltage-activated calcium channels that evoke small and transient calcium currents. Recently, T-type calcium channels have been implicated in neurodevelopmental disorders such as autism spectrum disorder and neural tube defects. However, their function during embryonic development is largely unknown. Here, we investigated the function and expression of T-type calcium channels in embryonic neural progenitor cells (NPCs). First, we compared the expression of T-type calcium channel subtypes (CaV3.1, 3.2, and 3.3) in NPCs and differentiated neural cells (neurons and astrocytes). We detected all subtypes in neurons but not in astrocytes. In NPCs, CaV3.1 was the dominant subtype, whereas CaV3.2 was weakly expressed, and CaV3.3 was not detected. Next, we determined CaV3.1 expression levels in the cortex during early brain development. Expression levels of CaV3.1 in the embryonic period were transiently decreased during the perinatal period and increased at postnatal day 11. We then pharmacologically blocked T-type calcium channels to determine the effects in neuronal cells. The blockade of T-type calcium channels reduced cell viability, and induced apoptotic cell death in NPCs but not in differentiated ...Continue Reading

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Citations

Jun 21, 2019·Journal of Medical Genetics·Norbert Weiss, Gerald W Zamponi
Oct 2, 2020·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasushi Yabuki
Jul 26, 2019·Neurobiology of Disease·Udaiyappan JanakiramanMark A Nelson

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

GraphPad Prism

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