T30177, an oligonucleotide stabilized by an intramolecular guanosine octet, is a potent inhibitor of laboratory strains and clinical isolates of human immunodeficiency virus type 1.

Antimicrobial Agents and Chemotherapy
J O OjwangT L Wallace

Abstract

T30177, an oligonucleotide composed of only deoxyguanosine and thymidine, is 17 nucleotides in length and contains single phosphorothioate internucleoside linkages at its 5' and 3' ends for stability. This oligonucleotide does not share significant primary sequence homology with or possess any complementary (antisense) sequence motifs to the human immunodeficiency virus type 1 (HIV-1) genome. T30177 inhibited replication of multiple laboratory strains of HIV-1 in human T-cell lines, peripheral blood lymphocytes, and macrophages. T30177 was also found to be capable of inhibiting multiple clinical isolates of HIV-1 and preventing the cytopathic effect of HIV-1 in primary CD4+ T lymphocytes. In assays with human peripheral blood lymphocytes there was no observable toxicity associated with T30177 at the highest concentration tested (100 microM), while the median inhibitory concentration was determined to be in the range of 0.1 to 1.0 microM for the clinical isolates tested, resulting in a high therapeutic index for this drug. In temporal studies, the kinetics of addition of T30177 to infected cell cultures indicated that, like the known viral adsorption blocking agents dextran sulfate and Chicago sky blue, T30177 needed to be added...Continue Reading

References

Jan 1, 1992·Antisense Research and Development·S Agrawal, J Y Tang
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W S MarshallM H Caruthers
Jan 11, 1992·Journal of Acquired Immune Deficiency Syndromes : JAIDS·D J ClantonJ P Bader
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J P BaderM R Boyd
Nov 1, 1990·AIDS Research and Human Retroviruses·V CiminaleG N Pavlakis
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M S McGrathJ Marsh
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R L LetsingerP S Sarin
Jul 1, 1985·Antimicrobial Agents and Chemotherapy·E De Clercq
Mar 31, 1988·Nature·P L Nara, P J Fischinger
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R WyattD J Ecker
Apr 1, 1994·Antimicrobial Agents and Chemotherapy·E De ClercqR Datema
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·A MazumderY Pommier
Feb 4, 1994·Journal of Molecular Biology·P SchultzeJ Feigon
Jul 9, 1993·Journal of Medicinal Chemistry·J F MilliganJ C Martin
Sep 1, 1993·Archives of Biochemistry and Biophysics·S CarteauC Auclair
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M R FesenY Pommier
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J LisziewiczS Agrawal

❮ Previous
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Citations

May 3, 2002·Reviews in Medical Virology·Vasu Nair
Jan 1, 1996·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·E De Clercq
Apr 29, 2000·Trends in Pharmacological Sciences·E De Clercq
Feb 26, 1998·Antiviral Research·E Asante-Appiah, A M Skalka
May 20, 1998·Antiviral Research·A K Field
Aug 15, 2002·Current Biology : CB·Christophe PannecouqueZeger Debyser
May 2, 2000·Drug Discovery Today·W S BlairO B Wallace
Nov 11, 2010·Nucleic Acids Research·Erik B PedersenVyacheslav V Filichev
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Robert F RandoHiroyasu Kokubo
Jun 8, 2001·Annual Review of Biochemistry·D M Eckert, P S Kim
Aug 20, 2014·Infectious Diseases and Therapy·Thibault Mesplède, Mark A Wainberg
Sep 25, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Domenica MusumeciDaniela Montesarchio
Mar 26, 2011·Expert Opinion on Biological Therapy·Donatella Rm NegriAndrea Cara
Sep 13, 2015·Biochimie·Mathieu MétifiotMarie-Line Andreola
May 16, 2007·Reviews in Medical Virology·Vasu Nair, Guochen Chi
Aug 23, 2008·Medicinal Research Reviews·Keduo QianKuo-Hsiung Lee
Apr 20, 2006·Antiviral Research·Arthur Van Aerschot
Sep 20, 2005·Virology·Mercedes Armand-UgónJosé A Esté
Mar 23, 2005·Virology·András HorváthJanos Aradi
Feb 7, 2001·Bioorganic Chemistry·K MaurerA D Leavitt
Aug 16, 2002·Antiviral Chemistry & Chemotherapy·Zeger DebyserMyriam Witvrouw
Dec 22, 2016·Pharmaceuticals·Víctor M GonzálezAna García-Sacristán
Mar 4, 2000·Bioorganic & Medicinal Chemistry Letters·M TaktakishviliV Nair
Aug 14, 2010·Viruses·Mathieu MétifiotYves Pommier
Jul 15, 2006·AIDS Patient Care and STDs·Max Lataillade, Michael J Kozal
Feb 13, 2018·RNA Biology·Jyoti BalaHoshang Unwalla
Jan 24, 2003·Expert Opinion on Therapeutic Targets·Khampoune SayasithJocelyn Yelle

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