T4 Pili Promote Colonization and Immune Evasion Phenotypes of Nonencapsulated M4 Streptococcus pyogenes.

MBio
Yi-Hsuan ChenYung-Chi Chang

Abstract

Streptococcus pyogenes (group A Streptococcus [GAS]) is an important human pathogen causing a broad spectrum of diseases and associated with significant global morbidity and mortality. Almost all GAS isolates express a surface hyaluronic acid capsule, a virulence determinant that facilitates host colonization and impedes phagocyte killing. However, recent epidemiologic surveillance has reported a sustained increase in both mucosal and invasive infections caused by nonencapsulated GAS, which questions the indispensable role of hyaluronic acid capsule in GAS pathogenesis. In this study, we found that pilus of M4 GAS not only significantly promotes biofilm formation, adherence, and cytotoxicity to human upper respiratory tract epithelial cells and keratinocytes, but also promotes survival in human whole blood and increased virulence in murine models of invasive infection. T4 antigen, the pilus backbone protein of M4 GAS, binds haptoglobin, an abundant human acute-phase protein upregulated upon infection and inflammation, on the bacterial surface. Haptoglobin sequestration reduces the susceptibility of nonencapsulated M4 GAS to antimicrobial peptides released from activated neutrophils and platelets. Our results reveal a previously...Continue Reading

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Citations

Feb 25, 2021·Scientific Reports·Jacelyn M S LohThomas Proft
Feb 27, 2021·Frontiers in Microbiology·Stefan Ness, Markus Hilleringmann
Feb 27, 2021·Frontiers in Microbiology·Masanobu Nakata, Bernd Kreikemeyer

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Datasets Mentioned

BETA
CP051138

Methods Mentioned

BETA
PCR
flow cytometry
pulldown
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism
CellQuest
ImageJ
FACSCalibur
GraphPad
GAS

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