T7 RNA polymerase transcription of Escherichia coli isoacceptors tRNA(Leu)

Science in China. Series C, Life Sciences
Y LiY L Wang

Abstract

The T7 promoter and the gene encoding tRNA(Leu) were synthesized chemically and cloned into plasmid pUC19, where the gene was transcribed in vitro with T7 RNA polymerase purified. It was found that spermidine had a negative effect on T7 transcription. The amount of transcribed tRNA was about 250 times that of the template DNA under optimal reaction conditions. The unmodified tRNA(1) (Leu) and tRNA(2) (Leu) had similar leucine accepting ability, which however was only one fourth that of the native modified ones. This result indicated that the modified nucleotides of tRNA(Leu) played some though not key roles in its aminoacylation.

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Citations

Jun 28, 2013·Nucleic Acids Research·Ru-Juan LiuEn-Duo Wang
Jun 23, 2009·Biochemical and Biophysical Research Communications·Xiao-Long Zhou, En-Duo Wang
Jul 27, 1999·Protein Expression and Purification·Y LiY Wang
Dec 13, 2018·Nucleic Acids Research·Jing LiRu-Juan Liu
Oct 29, 2008·The Journal of Biological Chemistry·Xiao-Long ZhouEn-Duo Wang
Dec 7, 2021·Nucleic Acids Research·Zhi-Xuan HuangRu-Juan Liu

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