TAAR1 induces a disturbed GSK3β phosphorylation in recurrent miscarriages through the ODC

Endocrine Connections
Stavroula StavrouAurelia Vattai

Abstract

Thyroid hormones play an important role in the maintenance of pregnancy. Their derivates, endogenous amines, act via binding to the trace amine-associated receptor (TAAR1). The aim of our study was to analyse the regulation of TAAR1, serine/threonine kinase (pGSK3β) and ornithine decarboxylase (ODC) in placentas of healthy pregnancies, spontaneous (SM) and recurrent miscarriages (RM) and to investigate the influence of thyroid hormone derivates on TAAR1 expression in trophoblast model cellsin vitro. Patients with SM (n = 15) and RM (n = 15) were compared with patients with healthy pregnancies (n = 15) (pregnancy weeks 7-13 each). Immunohistochemistry was applied to analyse placental TAAR1, pGSK3β and ODC expression. Protein expression of the receptors after stimulation with T3, T1AM and RO5203548 in BeWo trophoblast model cells was determined via Western blot. Double-immunofluorescence was used to determine placental expression of TAAR1 and ODC. Levels of TAAR1, pGSK3β and ODC were higher in placentas of RM in comparison to healthy controls. Stimulation of BeWo cells with T3, T1AM and RO5203548 significantly increased TAAR1 expression. ODC expression in BeWo cells was upregulated through T3. Via double-immunofluorescence, TAAR1...Continue Reading

Citations

Apr 25, 2018·Endocrine Connections·Michael J GratzAurelia Vattai
Jun 27, 2018·Pharmacological Reviews·Raul R GainetdinovMark D Berry
Jul 18, 2018·Frontiers in Pharmacology·Sherri L Christian, Mark D Berry
Dec 19, 2019·Cellular and Molecular Neurobiology·Zhengrong Xu, Qian Li
Dec 15, 2019·Cellular and Molecular Neurobiology·Katlynn Bugda GwiltGregory M Miller

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Methods Mentioned

BETA
PCR
FCS
protein assay

Software Mentioned

Bio
PowerPlex
SPSS Statistics
Rad Quantity One

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