Tacaribe virus Z protein interacts with the L polymerase protein to inhibit viral RNA synthesis

Journal of Virology
R JácamoMaria T Franze-Fernandez

Abstract

Tacaribe virus (TV) is the prototype of the New World group of arenaviruses. The TV genome encodes four proteins, the nucleoprotein (N), the glycoprotein precursor, the polymerase (L), and a small RING finger protein (Z). Using a reverse genetic system, we recently demonstrated that TV N and L are sufficient to drive transcription and full-cycle RNA replication mediated by TV-like RNAs and that Z is a powerful inhibitor of these processes (N. López, R. Jácamo, and M. T. Franze-Fernández, J. Virol. 65:12241-12251, 2001). In the present study we investigated whether Z might interact with either of the proteins, N and L, required for RNA synthesis. To that end, we used coimmunoprecipitation with monospecific antibodies against the viral proteins and coimmunoprecipitation with serum against glutathione S-transferase (GST) and binding to glutathione-Sepharose beads when Z was expressed as a fusion protein with GST. We demonstrated that Z interacted with L but not with N and that Z inhibitory activity was dependent on its ability to bind to L. We also evaluated the contribution of different Z regions to its binding ability and functional activity. We found that integrity of the RING structure is essential for Z binding to L and for Z...Continue Reading

References

May 1, 1991·Virology·S IapalucciM T Franze-Fernández
Jan 1, 1987·Current Topics in Microbiology and Immunology·D H Bishop, D D Auperin
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Jun 1, 1987·Virus Research·M T Franze-FernándezM M Zakin
May 1, 1996·Virology·M D BowenS T Nichol
Jul 1, 1996·Research in Virology·C RossiM T Franze-Fernández
Feb 2, 2000·Journal of Molecular Biology·K L Borden
Apr 18, 2000·The Journal of General Virology·N LópezM T Franze-Fernández

❮ Previous
Next ❯

Citations

May 29, 2008·Archives of Virology·Shuiyun LanYuying Liang
Apr 5, 2013·Archives of Virology·Frank LennartzWolfgang Garten
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Philip J Kranzusch, Sean P J Whelan
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
Jan 29, 2010·Journal of Virology·Allison GrosethStephan Becker
Oct 1, 2011·Journal of Virology·Maria Eugenia LoureiroNora Lopez
Nov 27, 2004·Journal of Virology·Meike HassStephan Günther
Mar 3, 2004·Journal of Virology·Tatjana I CornuJuan Carlos de la Torre
Jul 24, 2010·Journal of Biomedicine & Biotechnology·S E GoñiM E Lozano
Dec 4, 2012·Viruses·Sarah Katharina FehlingThomas Strecker
Feb 26, 2013·Viruses·Svenja WolffAllison Groseth
Oct 19, 2004·Critical Reviews in Clinical Laboratory Sciences·Stephan Günther, Oliver Lenz
May 23, 2012·Expert Opinion on Drug Discovery·Sheli R RadoshitzkySina Bavari
Jan 18, 2011·Microbes and Infection·Ricardo M GómezMirta Schattner
Jul 6, 2010·Antiviral Research·Claudia S SepúlvedaElsa B Damonte
Jan 15, 2005·Virus Research·Juan Carlos de la Torre
Apr 16, 2004·Virus Research·Maria N B Cajimat, Charles F Fulhorst
Nov 10, 2015·Scientific Reports·Raphaël Gaudin, Tomas Kirchhausen
Oct 30, 2016·Journal of Virology·Lei-Ke ZhangGengfu Xiao
Dec 29, 2005·The Journal of Biological Chemistry·Osvaldo ReyEnrique Rozengurt
Mar 31, 2019·The Journal of Biological Chemistry·Dominik VogelStephan Günther
May 16, 2017·PLoS Pathogens·Maria RosenthalSophia Reindl

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.