Tackling the methylome: recent methodological advances in genome-wide methylation profiling.

Genome Medicine
Marcos Rh Estécio, J P Issa

Abstract

DNA methylation of promoter CpG islands is strongly associated with gene silencing and is known as a frequent cause of loss of expression of tumor suppressor genes, as well as other genes involved in tumor formation. DNA methylation of driver genes is very likely outnumbered by the number of methylated passenger genes, though these can be useful as tumor markers. Much of what is known about the importance of DNA methylation in cancer was gained through small- and moderate-scale analysis of gene promoters and tumor samples. A much better understanding of the role of DNA methylation in cancer, either as a marker of disease or as an active driver of tumorigenesis, will likely be gained from genome-wide studies of this modification in normal and malignant cells. This goal has become more attainable with the recent introduction of large-scale genome analysis methodologies and these have been modified to allow for investigation of DNA methylation. Several research groups have been formed to coordinate efforts and apply these methodologies to decipher the methylome of healthy and diseased tissues. In this article we review technological advances in genome-wide methylation profiling.

Citations

Mar 31, 2011·Development, Growth & Differentiation·Christina Piperi, Athanasios G Papavassiliou
Jun 5, 2014·Journal of Molecular Cell Biology·Ahmad Besaratinia, Stella Tommasi
Dec 15, 2010·FEBS Letters·Marcos R H Estécio, Jean-Pierre J Issa
May 12, 2012·Current Opinion in Structural Biology·Jonas Korlach, Stephen W Turner
Sep 18, 2010·The Journal of Molecular Diagnostics : JMD·Floriana Della RagioneMaurizio D'Esposito
Mar 15, 2011·International Journal of Cancer. Journal International Du Cancer·Ryo NagashioYae Kanai
Aug 27, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Ahmad BesaratiniaStella Tommasi
Jan 3, 2014·The Journal of Clinical Investigation·Jean-Pierre Issa
Oct 22, 2010·Current Opinion in Endocrinology, Diabetes, and Obesity·Rocío M Rivera, Lynda B Bennett
Aug 15, 2013·Cancer Prevention Research·Tomomitsu TaharaJean-Pierre J Issa
Jan 6, 2011·Cancer Prevention Research·Jean-Pierre J Issa, Judy E Garber

❮ Previous
Next ❯

Methods Mentioned

BETA
deamination
PCR
Methyl-Seq
immunoprecipitation
pull-down
RRBS
MethylC-Seq

Software Mentioned

Solexa

Related Concepts

Related Feeds

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

© 2022 Meta ULC. All rights reserved