Taenia saginata metacestode antigenic fractions without affinity to concanavalin A are an important source of specific antigens for the diagnosis of human neurocysticercosis.

Clinical and Vaccine Immunology : CVI
Heliana B OliveiraJulia Maria Costa-Cruz

Abstract

Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin- and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64- to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this compo...Continue Reading

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Citations

Mar 14, 2013·Parasitology Research·Sameh AbuseirChristina Strube
Jun 26, 2012·BMJ Case Reports·Abraham Vincent Samuel JesudossAshish Rohatgi
Sep 18, 2010·Diagnostic Microbiology and Infectious Disease·Daniela da Silva NunesJulia Maria Costa-Cruz
Apr 26, 2011·Diagnostic Microbiology and Infectious Disease·Henrique Tomaz GonzagaJulia Maria Costa-Cruz
Aug 16, 2012·The Journal of Physiological Sciences : JPS·Isaac NaggarMark Stewart
Jun 14, 2020·Acta Parasitologica·Marwa A HassanAbeer Said Abdel-Ghany Al-Antably

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