TAFI, or plasma procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombin-thrombomodulin complex.

The Journal of Biological Chemistry
L BajzarM Nesheim


TAFI (thrombin-activatable fibrinolysis inhibitor) is a recently discovered plasma protein that can be activated by thrombin-catalyzed proteolysis to a carboxypeptidase B-like enzyme that inhibits fibrinolysis. This work shows that the thrombin-thrombomodulin complex, rather than free thrombin, is the most likely physiologic activator. Thrombomodulin increases the catalytic efficiency of the reaction by a factor of 1250, an effect expressed almost exclusively through an increase in kcat. The kinetics of the reaction conform to a model whereby thrombin can interact with either TAFI (Km = 1.0 microM) or thrombomodulin (Kd = 8.6 nM), and either binary complex so formed can then interact with the third component to form the ternary thrombin-thrombomodulin-TAFI complex from which activated TAFI is produced with kcat = 1.2 s-1. This work also shows that activated TAFI down-regulates tPA-induced fibrinolysis half-maximally at a concentration of 1.0 nM in a system of purified components. This concentration of TAFI is about 2% of the level of the zymogen in plasma, which indicates that ample activated TAFI could be generated to very significantly modulate fibrinolysis in vivo. Therefore, TAFI in vitro and possibly in vivo defines an exp...Continue Reading


Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B F Le Bonniec, C T Esmon
Jul 1, 1991·Seminars in Thrombosis and Hemostasis·T Astrup
Jan 1, 1991·Annals of the New York Academy of Sciences·C T EsmonN L Esmon
Jan 1, 1991·Annals of the New York Academy of Sciences·K G MannS Krishnaswamy
Jan 1, 1990·Advances in Experimental Medicine and Biology·N J de FouwR M Bertina
Apr 23, 1990·Biochimica Et Biophysica Acta·D HendriksM van Sande
Feb 15, 1987·Thrombosis Research·M D Burdick, R G Schaub
Mar 15, 1985·Thrombosis Research·F B Taylor, M S Lockhart
May 11, 1982·Biochemistry·L S HibbardK G Mann
Nov 1, 1995·The Journal of Clinical Investigation·A RedlitzE F Plow
Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C T Esmon
Jun 16, 1995·The Journal of Biological Chemistry·L BajzarM E Nesheim

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