TagFinder: preprocessing software for the fingerprinting and the profiling of gas chromatography-mass spectrometry based metabolome analyses

Methods in Molecular Biology
Alexander LuedemannJoachim Kopka

Abstract

GC-MS based metabolome studies aim for the complete identification and relative or absolute quantification of metabolites in complex extracts from a large diversity of biological materials. The resulting high-throughput chromatography data files are typically processed following two complementary workflows, namely, fingerprinting and profiling. For fingerprinting studies all observed mass features, here called mass spectral tags (MSTs), are quantified in a nontargeted and (within the limits of the GC-MS technology) comprehensive approach. Fingerprinting allows for the discovery of MSTs, which, in the sense of a biomarker, indicate significant changes of metabolite pool sizes. The significance and relevance of such MSTs are typically tested in comparison to standardized reference samples. Only after this confirmation step are the relevant MSTs identified and the underlying metabolic biomarkers elucidated. Both the metabolite fingerprinting and profiling approaches are essential to modern biotechnological investigations. Studies which are aimed at establishing the substantial equivalence at metabolic level or aim to breed for optimum quality of human food or animal feed especially benefit from the potential to discover novel unfo...Continue Reading

Citations

Mar 15, 2018·Journal of Experimental Botany·Mariette AnderssonPer Hofvander
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