Tailor-made core-shell nanospheres for antisense oligonucleotide delivery: IV. Adsorption/release behaviour

Journal of Biomaterials Science. Polymer Edition
L TondelliM Laus

Abstract

The adsorption/release behaviour of oligodeoxynucleotides (ODNs) on double functional core-shell polymethylmethacrylate nanospheres, with a narrow size distribution, is described. The outer shell consists of alkyl or glycolic chains containing permanently-charged quaternary ammonium groups. Ion pair formation between negatively-charged ODN phosphate groups and positively-charged groups, present on the nanosphere surface, is the main mechanism of interaction. The amount of adsorbed ODN depends on both the ODN concentration and the nanosphere surface charge density. An adsorption-induced swelling mechanism is proposed in which a modification of the charged diffuse layer around the nanospheres increases the ODN binding site accessibility with increasing ODN concentration. Adsorption on the nanosphere surface prevents serum degradation of the ODNs. ODN release is negligible in the presence of culture medium but occurs gradually in the presence of serum. No significant cytotoxicity of the free nanoparticles was found in PBMC and CEM cells after 24 h at ODN concentrations required for antisense activity.

References

Apr 17, 1992·Biochimica Et Biophysica Acta·A K SinghE J Lewis
Aug 30, 1991·Biochemical and Biophysical Research Communications·X Gao, L Huang
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Apr 1, 1995·The Journal of Clinical Investigation·C BeltingerA M Gewirtz
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·G SchwabT Saison-Behmoaras
Jan 1, 1997·Trends in Pharmacological Sciences·S Akhtar, S Agrawal
Nov 15, 1997·Antisense & Nucleic Acid Drug Development·H P ZobelA Zimmer
Sep 22, 1998·Antisense & Nucleic Acid Drug Development·S T Crooke
Feb 1, 2000·Molecular Medicine Today·S Agrawal, E R Kandimalla
May 9, 2000·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·H P ZobelA Zimmer
Jul 18, 2000·Journal of the American College of Surgeons·D W GreenJ A Drebin

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Citations

Feb 11, 2004·Journal of Biomaterials Science. Polymer Edition·Luisa TondelliMichele Laus

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