Tailoring of horseradish peroxidase activity in cationic water-in-oil microemulsions

Langmuir : the ACS Journal of Surfaces and Colloids
Sangita RoyPrasanta Kumar Das

Abstract

Horseradish peroxidase (HRP) in cationic water-in-oil (W/O) microemulsions has always been ignored in reverse micellar enzymology, mainly because cationic surfactants are inhibitors of enzyme peroxidase. In the present study, for the first time, we have successfully introduced the cationic W/O microemulsion as an attractive host for efficient HRP activity. To this notion, much improved activity of HRP was observed in the W/O microemulsion of cetyltrimethylammonium bromide (CTAB) with an increase in n-hexanol concentration and W0 ([water]/[surfactant]), presumably due to the increased interfacial area of the microemulsions. In support of our above observation, six surfactants were synthesized with an increased headgroup size where the methyl groups of CTAB were subsequently replaced by the n-propyl and 2-hydroxyethyl groups, respectively, to prepare mono-, di-, and tripropylated/hydroxyethylated n-hexadecylammonium bromide. The peroxidase activity enhanced with headgroup size and also followed an overall trend similar to that found in the case of CTAB. Possibly, the reduced positive charge density at the augmented interfacial area by means of increase, either in headgroup size, cosurfactant concentration, and/or W0, is not capab...Continue Reading

References

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Jun 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Sekh MahiuddinWerner Kunz
Aug 23, 2005·Journal of Colloid and Interface Science·Antara DasguptaPrasanta Kumar Das
Dec 14, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Rajendra Narayan MitraPrasanta Kumar Das
Jun 27, 2007·Biotechnology and Bioengineering·Yuchun XieAlexander M Klibanov

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Citations

Dec 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Moumita GhoshPrasanta Kumar Das
Jan 29, 2013·Colloids and Surfaces. B, Biointerfaces·Luyan XueYinbo Qu
Mar 14, 2007·Biochemical and Biophysical Research Communications·Sisir DebnathPrasanta Kumar Das
Dec 17, 2009·Chemistry : a European Journal·Subhabrata MaitiPrasanta Kumar Das
Dec 31, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·M MoniruzzamanM Goto
Jan 24, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Weican ZhangPeiji Gao
Feb 28, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Dibyendu Das, Prasanta Kumar Das
Feb 15, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Evangelia D TzikaAristotelis Xenakis
Oct 4, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Sisir DebnathPrasanta Kumar Das
Mar 14, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Anshupriya ShomePrasanta Kumar Das

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