Tailoring the gelatin/chitosan electrospun scaffold for application in skin tissue engineering: an in vitro study

Progress in Biomaterials
Mohamad Pezeshki-ModaressSarah Rajabi

Abstract

The nanofibrous structure containing protein and polysaccharide has good potential in tissue engineering. The present work aims to study the role of chitosan in gelatin/chitosan nanofibrous scaffolds fabricated through electrospinning process under optimized condition. The performance of chitosan in gelatin/chitosan nanofibrous scaffolds was evaluated by mechanical tests, scanning electron microscopy (SEM), Fourier transform infrared (FTIR) and in vitro cell culture on scaffolds with different gelatin/chitosan blend ratios. To assay the influence of chitosan ratio on biocompatibility of the electrospun gelatin/chitosan scaffolds for skin tissue engineering, the culturing of the human dermal fibroblast cells (HDF) on nanofibers in terms of attachment, morphology and proliferation was evaluated. Morphological observation showed that HDF cells were attached and spread well on highly porous gelatin/chitosan nanofibrous scaffolds displaying spindle-like shapes and stretching. The fibrous morphologies of electrospun gelatin/chitosan scaffolds in culture medium were maintained during 7 days. Cell proliferation on electrospun gelatin/chitosan scaffolds was quantified by MTS assay, which revealed the positive effect of chitosan content ...Continue Reading

References

Dec 31, 2002·Biomaterials·Jin Shu MaoKang De Yao
Jan 11, 2003·Journal of Biomedical Materials Research. Part a·Jinshu MaoYilin Cao
Jan 25, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tzu-Wei WangFeng-Huei Lin
Oct 6, 2007·Advanced Drug Delivery Reviews·Catherine P BarnesGary L Bowlin
Apr 12, 2008·Biomaterials·Sepideh Heydarkhan-HagvallWilliam R MacLellan
Jan 27, 2010·Biotechnology Advances·Nandana Bhardwaj, Subhas C Kundu
Jul 10, 2010·Journal of Biomaterials Science. Polymer Edition·Yong-Fang QianXiu-Mei Mo
Jan 26, 2011·Biotechnology Advances·R JayakumarH Tamura
Jun 10, 2011·Bio-medical Materials and Engineering·Javad JafariFazel Gorjipour
Aug 27, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mansor Bin AhmadMei Yen Tay
Mar 10, 2012·International Journal of Nanomedicine·Jui-Yang LaiTing-Chun Yu
Dec 11, 2013·Journal of Biomedical Materials Research. Part a·Mohamad Pezeshki-ModaressNasser Aghdami
Aug 9, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fatemeh MottaghitalabMehdi Farokhi
Sep 3, 2016·Journal of Biomedical Materials Research. Part a·Mohamad Pezeshki-ModaressMohammad R K Mofrad
Apr 9, 2018·International Journal of Biological Macromolecules·Amin SadeghiMojgan Zandi

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Citations

Jun 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandra Elena StoicaAlexandru Mihai Grumezescu
Jul 22, 2019·Journal of Functional Biomaterials·Jimna Mohamed AmeerNaresh Kasoju
Apr 13, 2019·Polymers·Patrícia AlvesPaula Ferreira
Jan 29, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad Umar Aslam KhanRashid Amin
Jan 30, 2021·International Journal of Biological Macromolecules·Soroush AhmadiSeyedeh Sara Ashraf
Feb 23, 2020·Bioactive Materials·Md Minhajul IslamTaslim Ur Rashid
Dec 4, 2020·Tissue Engineering. Part B, Reviews·Min JinDong-An Wang
Oct 15, 2020·Journal of Wound Care·Seyedeh-Sara HashemiMehrzad Jafari Barmak

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Methods Mentioned

BETA
scanning electron microscopy
infrared spectroscopy

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