Tailoring the mechanical properties of gelatin methacryloyl hydrogels through manipulation of the photocrosslinking conditions

Soft Matter
Cathal D O'ConnellGordon G Wallace

Abstract

Photo-crosslinkable hydrogels, in particular gelatin methacryloyl (GelMa), are gaining increasing importance in biofabrication and tissue engineering. While GelMa is often described as mechanically 'tunable', clear relationships linking the photocrosslinking conditions to reaction rates, and the resulting mechanical properties, have not been described. Meanwhile the conditions employed in the literature are disparate, and difficult to compare. In this work, in situ rheological measurements were used to quantify the relative rate of reaction of GelMa hydrogels with respect to light intensity, exposure time and photo-initiator concentration. In addition the UV degradation of the photo-initiator Irgacure 2959 was measured by UV-vis spectroscopy, and used to estimate the rate of free radical production as a function of light exposure. Using these data an expression was derived which predicts the mechanical properties of GelMa hydrogels produced across a wide range of crosslinking conditions. The model was validated through fabrication of a GelMa gradient which matched predicted properties. Human mesenchymal stem cells encapsulated in crosslinked GelMa exhibited high (>90%) viability post encapsulation, however metabolic activity ov...Continue Reading

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Citations

Aug 23, 2018·Soft Matter·Andres RulandGordon G Wallace
Jul 12, 2019·Biofabrication·Sebastian Eggert, Dietmar W Hutmacher
Feb 25, 2020·Macromolecular Rapid Communications·Danielle BruenLarisa Florea
May 28, 2020·Journal of the American Heart Association·Leon M PtaszekJeremy N Ruskin
Oct 3, 2020·Bioengineering & Translational Medicine·Nicole ZorattoAmir Sheikhi
Jun 17, 2020·Macromolecular Bioscience·Antonina LavrentievaIliyana Pepelanova
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Oct 5, 2019·Materials·Natasha Antill-O'BrienCathal D O'Connell
Oct 8, 2020·Advanced Healthcare Materials·Amelie ErbenMichael Heymann
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Mar 5, 2021·Biofabrication·Maria Del Rocio Garcia CruzLaurence Meagher
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Methods Mentioned

BETA
bioprinting
rheology
AFM
confocal microscopy
fluorescence imaging

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