Tamoxifen administration to mice

Cold Spring Harbor Protocols
Jonathan WhitfieldLaura Soucek

Abstract

The strategy of fusing a protein of interest to a hormone-binding domain (HBD) of a steroid hormone receptor allows fine control of the activity of the fused protein. Such fusion proteins are inactive in the absence of ligand, because they are complexed with a variety of intracellular polypeptides. Upon ligand binding, the receptor is released from its inhibitory complex and the fusion protein becomes functional. In the murine estrogen receptor (ER) fusion system, proteins are fused to the HBD of the ER. The system relies on the use of a mutant ER known as ER(TAM). Compared to the wild-type HBD, ER(TAM) has 1000-fold lower affinity for estrogen, yet remains responsive to activation by the synthetic steroid 4-hydroxytamoxifen (4-OHT). Because 4-OHT is expensive, animals can be treated with the cheaper precursor tamoxifen, which is converted into 4-OHT by a liver enzyme. Here we present an overview of the methods used to deliver tamoxifen to mice. The most used method is intraperitoneal injection, because the amount of administered compound can be better controlled, but delivery by oral gavage is also possible. For short-term and immediate-effect studies or when conversion of tamoxifen by the liver is to be avoided, 4-OHT can be ...Continue Reading

References

Jan 12, 2007·Genesis : the Journal of Genetics and Development·Claudia KiermayerMarkus Brielmeier
Mar 4, 2015·Cold Spring Harbor Protocols·Jonathan WhitfieldLaura Soucek

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Citations

Apr 5, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rudragouda ChannappanavarStanley Perlman
Jan 13, 2018·Science·Irina LeonardiIliyan D Iliev
Jul 11, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Andrew S HaganDavid M Ornitz
Feb 28, 2020·Journal of Applied Physiology·C Brooks MobleyJohn J McCarthy
Mar 11, 2020·Frontiers in Oncology·Florian GabelYannick Goumon
Sep 19, 2020·Scientific Reports·Rachel S DonocoffRemi J Creusot
Apr 29, 2020·International Journal of Molecular Sciences·Yasmin M AttiaGabriel Lopez-Berestein
Mar 4, 2015·Cold Spring Harbor Protocols·Jonathan WhitfieldLaura Soucek

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