Tamoxifen for induction of Cre-recombination may confound fibrosis studies in female mice

Journal of Cell Communication and Signaling
Lucas L FalkeRoel Goldschmeding

Abstract

A variety of conditional knock-out mice relying on Tamoxifen-driven ERT2/Cre -mediated recombination are available and have been used to study involvement of specific genes in kidney disease. However, recent data suggest that Tamoxifen itself might attenuate fibrosis when administered during experimental models of kidney disease. It has remained unclear whether this still applies also if kidney damage is initiated after a wash-out period has been implemented. Here we report that the commonly applied regimen of administration of 4 alternate day doses of 1mg Tamoxifen per mouse until 14 days prior to start of the actual experiment, in this case the induction of obstructive nephropathy by Unilateral Ureteral Obstruction (UUO), still attenuated fibrosis in female obstructed mouse kidneys, whereas this effect was not seen in male obstructed kidneys. Attenuation of fibrosis was accompanied by a reduction in nuclear ERα positivity despite absence of detectable levels of the active tamoxifen metabolite endoxifen throughout the UUO experiment. In conclusion, these results indicate that the Tamoxifen dosing regimen commonly applied in conditional gene targeting experiments might have prolonged confounding effects in female mice through a...Continue Reading

References

May 25, 2005·Contributions to Nephrology·Mohammed S RazzaqueTakashi Taguchi
Nov 2, 2005·Breast Cancer Research and Treatment·D J BrittonR I Nicholson
Feb 15, 2008·Annual Review of Physiology·Eric R ProssnitzHelen J Hathaway
Sep 18, 2008·International Journal of Cancer. Journal International Du Cancer·Pagona LagiouDimitrios Trichopoulos
Aug 25, 2009·Nature Reviews. Cancer·Jianming XuBert W O'Malley
Aug 25, 2009·Nature Reviews. Cancer·Elizabeth A Musgrove, Robert L Sutherland
May 25, 2011·Breast Cancer Research and Treatment·Atanas IgnatovThomas Kalinski
Nov 5, 2011·Journal of the American Society of Nephrology : JASN·Humberto DellêIrene L Noronha
Aug 14, 2013·PloS One·Debra L IrsikPascale H Lane
Sep 26, 2013·Breast Cancer Research and Treatment·Kathryn J RuddyHarold J Burstein
May 31, 2014·Cell Research·Qin FengBert W O'Malley
Jun 14, 2014·Experimental and Therapeutic Medicine·Shanhua MaoHaowen Jiang
Jul 18, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Dal KimKyung Pyo Kang
Jun 23, 2015·Journal of Cellular Physiology·Jon M CarthyAristidis Moustakas
Mar 2, 2016·Hypertension·Ko-Ting LuCurt D Sigmund
Mar 18, 2016·Clinical Kidney Journal·Floor E van der BiltEric F H van Bommel

❮ Previous
Next ❯

Citations

Jun 20, 2018·The Journal of Clinical Investigation·Shin-Ichi NurekiMichael F Beers
Feb 2, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Liang ZhaoMin Du
Oct 25, 2020·Pigment Cell & Melanoma Research·Gennie L ParkmanSheri L Holmen
Nov 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Stine Julie TingskovRikke Nørregaard
Apr 5, 2021·Kidney International·Szu-Yu PanShuei-Liong Lin
Apr 15, 2021·Scientific Reports·Kevin M BlumChristopher K Breuer

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
nuclear translocation

Software Mentioned

GraphPad Prism
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.