Tamoxifen improves glucose tolerance in a delivery, sex, and strain-dependent manner in mice

BioRxiv : the Preprint Server for Biology
Alexis M CeasrineRejji Kuruvilla

Abstract

Tamoxifen, a selective estrogen receptor modulator, is widely used in mouse models to temporally control gene expression but is also known to affect body composition. Here, we report that tamoxifen has significant and sustained effects on glucose tolerance, independent of effects on insulin sensitivity, in mice. Intraperitoneal, but not oral, tamoxifen delivery improved glucose tolerance in three inbred mouse strains. The extent and persistence of tamoxifen-induced effects were sex- and strain-dependent. These findings highlight the need to revise commonly used tamoxifen-based protocols for gene manipulation in mice by including longer chase periods following injection, oral delivery, and the use of tamoxifen-treated littermate controls.

Related Concepts

Gene Expression
Genetic Engineering
Insulin
Mice, Inbred A
Laboratory mice
Tamoxifen
Glucose Tolerance
Oral Cavity
Intraperitoneal
Estrogen Receptor Modulators

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