Tamoxifen-inducible podocyte-specific iCre recombinase transgenic mouse provides a simple approach for modulation of podocytes in vivo.

Genesis : the Journal of Genetics and Development
Jinrong WangFarhad R Danesh

Abstract

We report the generation and initial characterization of a mouse line expressing tamoxifen-inducible improved Cre (iCre) recombinase (iCre-ER(T2)) under the regulation of NPHS2 (podocin) gene promoter. The resulting transgenic mouse line was named podocin-iCreER(T2) mice. The efficiency of iCre activity was confirmed by crossing podocin-iCreER(T2) with the ROSA26 reporter mouse. By using the floxed ROSA reporter mice, we found that tamoxifen specifically induced recombination in the kidneys. In the absence of tamoxifen, recombination was undetectable in podocin-iCreER(T2);ROSA26 mice. However, following intraperitoneal injection of tamoxifen, selective recombination was observed in the podocytes of adult animals. We further examined the efficiency of recombination by assessing various tamoxifen exposure regimens in adult mice. These results suggest that podocin-iCre-ER(T2) mouse provides an excellent genetic tool to examine the function of candidate genes in podocytes in a spatially and temporally-restricted manner.

References

Aug 28, 1997·Biochemical and Biophysical Research Communications·R FeilP Chambon
Jun 3, 1998·Methods : a Companion to Methods in Enzymology·B Sauer
May 1, 2001·Methods : a Companion to Methods in Enzymology·D Metzger, P Chambon
Feb 9, 2002·Genesis : the Journal of Genetics and Development·D R ShimshekR Sprengel
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Vera EreminaSusan E Quaggin
Dec 14, 2002·Genesis : the Journal of Genetics and Development·Marcus J MoellerLawrence B Holzman
Feb 22, 2003·Journal of the American Society of Nephrology : JASN·Laurence BugeonMargaret J Dallman
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Tetsuya ShigeharaJeffrey B Kopp
Mar 24, 2005·American Journal of Physiology. Renal Physiology·Marcus J MoellerLawrence B Holzman
Feb 10, 2006·Journal of the American Society of Nephrology : JASN·Juuso JuhilaHarry Holthofer
Jan 11, 2007·Genesis : the Journal of Genetics and Development·Mo ChenDi Chen

❮ Previous
Next ❯

Citations

Aug 12, 2014·The American Journal of Pathology·Kan-Ichiro IharaKatsuhiko Nishimori
Jun 24, 2014·The American Journal of Pathology·Peter Y ChuangJohn C He
Dec 19, 2012·The American Journal of Pathology·Martin HöhneThomas Benzing
Mar 21, 2017·Scientific Reports·Lauren E WoodardMatthew H Wilson
Jan 24, 2018·American Journal of Physiology. Renal Physiology·Lihe ChenWenzheng Zhang
Apr 14, 2017·American Journal of Physiology. Renal Physiology·Mixuan YiZheng Dong
Jan 25, 2018·American Journal of Physiology. Renal Physiology·Huizhen WangWenjian Wang
Jun 29, 2016·Nature Communications·Shawn S BadalFarhad R Danesh
May 27, 2010·The Journal of Biological Chemistry·Jianyin LongFarhad R Danesh
Jan 31, 2016·Journal of the American Society of Nephrology : JASN·Bernard A AyangaFarhad R Danesh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.