TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana

Frontiers in Plant Science
Fengtao WangShichang Xu

Abstract

Plant-specific NAC transcription factors (TFs) constitute a large family and play important roles in regulating plant developmental processes and responses to environmental stresses, but only some of them have been investigated for effects on disease reaction in cereal crops. Virus-induced gene silencing (VIGS) is an effective strategy for rapid functional analysis of genes in plant tissues. In this study, TaNAC1, encoding a new member of the NAC1 subgroup, was cloned from bread wheat and characterized. It is a TF localized in the cell nucleus, and contains an activation domain in its C-terminal. TaNAC1 was strongly expressed in wheat roots and was involved in responses to infection by the obligate pathogen Puccinia striiformis f. sp. tritici and defense-related hormone treatments such as salicylic acid (SA), methyl jasmonate, and ethylene. Knockdown of TaNAC1 with barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) enhanced stripe rust resistance. TaNAC1-overexpression in Arabidopsis thaliana plants gave enhanced susceptibility, attenuated systemic-acquired resistance to Pseudomonas syringae DC3000, and promoted lateral root development. Jasmonic acid-signaling pathway genes PDF1.2 and ORA59 were constitutively expre...Continue Reading

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Citations

Mar 2, 2019·Plant Biotechnology Journal·Alexandre PerochonFiona M Doohan
Apr 14, 2019·Planta·Pramod PrasadSubodh Kumar
Jan 8, 2020·International Journal of Molecular Sciences·Xuan Lan Thi HoangNguyen Phuong Thao
Dec 12, 2020·Plant Biotechnology Journal·Lisha ShenAimin Zhang
Dec 31, 2020·International Journal of Molecular Sciences·Zhiyuan BianChongying Wang

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Methods Mentioned

BETA
transgenic
reverse-transcription PCR
PCR
one-hybrid
genetic interference
transgenics

Software Mentioned

PROSITE Scan
TBLASTX
MEGA4
DNAMAN
SPSS
BLAST
Clustal X
ORF Finder
ABI PRISM 7500

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