TaNAC69 from the NAC superfamily of transcription factors is up-regulated by abiotic stresses in wheat and recognises two consensus DNA-binding sequences

Functional Plant Biology : FPB
Gang-Ping XueRay Shorter

Abstract

NAC proteins are one of the largest families of plant transcription factors and have recently been implicated in diverse physiological processes. To elucidate their role in gene regulation, we determined the DNA-binding specificity of a drought- and cold-inducible NAC protein, TaNAC69 from wheat, and analysed its homologues from other species. Two consensus DNA-binding sequences (spanning 23-24 bp) of TaNAC69 were identified through binding site selection and both consisted of two half sites. Comprehensive data on the DNA-binding specificity of TaNAC69 were generated through extensive base substitution mutagenesis. TaNAC69 and its homologue in Arabidopsis, NAP, sharing 75% sequence identity in the NAC domain, exhibited similar DNA-binding specificity. TaNAC69 was able to homodimerise through its NAC domain. The NAC domain consists of five conserved subdomains. Subdomain mutation showed that a loss or reduction in TaNAC69 dimerisation capacity was accompanied with abolition or decrease in its DNA-binding activity. These data suggest that all subdomains are necessary to maintain a functional NAC domain structure required for interaction with DNA and dimerisation.

References

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Citations

Feb 18, 2011·Functional & Integrative Genomics·Troy J StephensonGang-Ping Xue
Aug 16, 2011·The Plant Journal : for Cell and Molecular Biology·Gang-Ping XueC Lynne McIntyre
Apr 23, 2015·Applied Biochemistry and Biotechnology·Farhad Masoomi-AladizgehKhadijeh Mozaffari
Oct 10, 2019·The New Phytologist·Vikas DevkarSalma Balazadeh
Apr 1, 2007·Functional Plant Biology : FPB·Juan Jose Olivares-VillegasGlenn K McDonald
Jul 13, 2017·G3 : Genes - Genomes - Genetics·Philippa BorrillCristobal Uauy
Apr 20, 2019·Journal of Experimental Botany·Xuemin MaBernd Mueller-Roeber
Jul 11, 2019·International Journal of Molecular Sciences·Shahbaz KhanZhi-Qiang Gao
Dec 12, 2020·Plant Biotechnology Journal·Lisha ShenAimin Zhang
Dec 17, 2009·Journal of Plant Physiology·Catherine JamarPatrick du Jardin

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Datasets Mentioned

BETA
AY625682

Methods Mentioned

BETA
transgenic
electrophoretic mobility shift
PCR
electrophoresis
size-exclusion high-performance chromatography
subunit exchange assay
affinity purification
gel filtration
pull-down
subunit exchange

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