Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination.

Scientific Reports
Lin ZhangXiaoming Ding

Abstract

We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks.

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Citations

Apr 16, 2013·Applied Microbiology and Biotechnology·Maksym Myronovskyi, Andriy Luzhetskyy
Oct 31, 2012·Biotechnology Letters·Amit Kumar ChaudharyJae Kyung Sohng
Oct 16, 2013·Journal of Industrial Microbiology & Biotechnology·Ryan E CobbHuimin Zhao
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Feb 8, 2019·Biotechnology and Bioengineering·Liping WangYunzi Luo
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Jul 2, 2017·Nucleic Acids Research·Femi J OlorunnijiW Marshall Stark
Nov 20, 2016·Acta Biochimica Et Biophysica Sinica·Xianwei WangXiaoming Ding
Dec 23, 2016·Applied and Environmental Microbiology·Paul C M FoggMargaret C M Smith
Jun 15, 2019·Natural Product Reports·Jia Jia ZhangBradley S Moore
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Sep 10, 2015·Microbiology Spectrum·Margaret C M Smith
Jun 1, 2021·FEMS Microbiology Letters·Rainer BreitlingEriko Takano
Jan 11, 2018·ACS Synthetic Biology·Christine A MerrickSusan J Rosser

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SSRTA
[UNK]
BioBrick
SSRAT

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