Tandem genes of Chlamydia psittaci that encode proteins localized to the inclusion membrane

Molecular Microbiology
J P BannantineT Hackstadt

Abstract

Chlamydiae are obligate intracellular bacteria that replicate within a non-acidified vacuole, termed an inclusion. To identify chlamydial proteins that are unique to the intracellular phase of the life cycle, a lambda expression library of Chlamydia psittaci DNA was differentially screened with convalescent antisera from infected guinea pigs and antisera directed at formalin-fixed purified chlamydial elementary bodies (EBs). One library clone was identified that harboured two open reading frames (ORFs) with coding potential for similar-sized proteins of approximately 20 kDa. These proteins were subsequently termed IncB and IncC. Sequencing of the cloned insert revealed a strong Escherichia coli-like promoter sequence immediately upstream of incB and a 36nt intergenic region between the ORFs. Sequence analysis of the region upstream of incB and incC revealed two ORFs that had strong homologies to an amino acid transporter and a sodium-dependent transporter. Immunoblotting with antisera directed at IncB or IncC demonstrated that these proteins are present in C. psittaci-infected HeLa cells but are absent or below the level of detection in purified EBs. Reverse transcriptase-polymerase chain reactions provided evidence that incB a...Continue Reading

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Citations

Nov 1, 2003·Medical Microbiology and Immunology·R LugertU Gross
Oct 5, 2001·FEMS Microbiology Letters·L DaugaardS Birkelund
Mar 23, 2002·Microbes and Infection·Daniel D RockeyWendy J Brown
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Sep 19, 2000·Infection and Immunity·D D RockeyR S Stephens
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May 6, 2003·Journal of Bacteriology·Tracy L NicholsonRichard S Stephens
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Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kenneth A Fields, Ted Hackstadt
Jul 3, 2004·BMC Microbiology·Damir AlzhanovDaniel D Rockey
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Dec 21, 2000·Molecular Microbiology·K A Fields, T Hackstadt

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