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Tandem immunoaffinity chromatography for plasma 1 alpha,25-dihydroxyvitamin D3 utilizing two antibodies having different specificities: a novel and powerful pretreatment tool for 1 alpha,25-dihydroxyvitamin D3 radioreceptor assays

The Journal of Steroid Biochemistry and Molecular Biology

Sep 1, 1995

N KobayashiK Shimada

PMID: 7577703

Abstract

We report here a novel and powerful pretreatment method for radioreceptor assays (RRAs) for human plasma 1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) based on "Tandem" immunoaffinity chromatography (Tandem IAC). Two antibodies having different specificities were each immobilized on agar...read more

Mentioned in this Paper

Human plasma
VDR gene
Gas Chromatography-Mass Spectrometry
Ethers
Anesthetic ether
Bromides Measurement
Soltriol
Tandem
Immunoaffinity Chromatography
Kidney Failure, Chronic
Paper Details
References
  • References22
  • Citations2
123

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Tandem immunoaffinity chromatography for plasma 1 alpha,25-dihydroxyvitamin D3 utilizing two antibodies having different specificities: a novel and powerful pretreatment tool for 1 alpha,25-dihydroxyvitamin D3 radioreceptor assays

The Journal of Steroid Biochemistry and Molecular Biology

Sep 1, 1995

N KobayashiK Shimada

PMID: 7577703

DOI:

Abstract

We report here a novel and powerful pretreatment method for radioreceptor assays (RRAs) for human plasma 1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) based on "Tandem" immunoaffinity chromatography (Tandem IAC). Two antibodies having different specificities were each immobilized on agar...read more

Mentioned in this Paper

Human plasma
VDR gene
Gas Chromatography-Mass Spectrometry
Ethers
Anesthetic ether
Bromides Measurement
Soltriol
Tandem
Immunoaffinity Chromatography
Kidney Failure, Chronic

Trending Feeds

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Related Papers

The Journal of Steroid Biochemistry and Molecular Biology

Enzyme immunoassay for plasma 25-hydroxyvitamin D3 employing immunoaffinity chromatography as a pretreatment method

The Journal of Steroid Biochemistry and Molecular BiologyJanuary 1, 1993
N KobayashiK Shimada
Paper Details
References
  • References22
  • Citations2
123
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