Tandem mass spectra of tryptic peptides at signal-to-background ratios approaching unity using electrospray ionization high-field asymmetric waveform ion mobility spectrometry/hybrid quadrupole time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
David A BarnettRoger Guevremont

Abstract

High-field asymmetric waveform ion mobility spectrometry (FAIMS) has been coupled to a quadrupole time-of-flight mass spectrometer for the tandem mass spectrometric analysis of tryptic peptides of pig hemoglobin. Using FAIMS, low levels (fmol/microL) of multiply charged tryptic peptides were separated from relatively intense chemical background such that their tandem mass spectra (MS/MS) lacked many background-related fragment ions observed using a conventional ESI-QqTOFMS instrument. Substantial improvements in both first-order and tandem mass spectra were realized while maintaining approximately the same absolute intensities.

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Citations

Nov 22, 2002·Journal of the American Society for Mass Spectrometry·David A BarnettRandy W Purves
Aug 28, 2009·Analytical Chemistry·Alexandre A ShvartsburgDanielle Toutoungi
Dec 22, 2011·Molecular & Cellular Proteomics : MCP·Kristian E SwearingenRobert L Moritz
Feb 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Markus KalkumBrian T Chait
Dec 1, 2012·Expert Review of Proteomics·Kristian E Swearingen, Robert L Moritz
Feb 5, 2016·Journal of the American Society for Mass Spectrometry·Helen J Cooper
Nov 26, 2015·Journal of Chromatography. a·Michael A EwingDavid E Clemmer
Jun 15, 2011·Methods : a Companion to Methods in Enzymology·Sophie R HarveyPerdita E Barran
Jul 12, 2002·Journal of Mass Spectrometry : JMS
Apr 7, 2018·Journal of the American Society for Mass Spectrometry·Sibylle PfammatterPierre Thibault
Dec 15, 2004·Analytical Chemistry·Alexandre A ShvartsburgRichard D Smith
Oct 27, 2006·The Journal of Physical Chemistry. B·Alexandre A ShvartsburgRichard D Smith

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