Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows.

Scientific Reports
Shveta BathlaAshok Kumar Mohanty

Abstract

Bovine milk is vital for infant nutrition and is a major component of the human diet. Bovine mastitis is a common inflammatory disease of mammary gland in cattle. It alters the immune profile of the animal and lowers the quality and yield of milk causing huge economic losses to dairy industry. The incidence of sub-clinical mastitis (SCM) is higher (25-65% worldwide) than clinical mastitis (CM) (>5%), and frequently progresses to clinical stage due to lack of sensitive and specific detection method. We used quantitative proteomics to identify changes in milk during sub-clinical mastitis, which may be potential biomarkers for developing rapid, non-invasive, sensitive detection methods. We performed comparative proteome analysis of the bovine milk, collected from the Indian hybrid cow Karan Fries. The differential proteome in the milk of Indian crossbred cows during sub-acute and clinical intramammary gland infection has not been investigated to date. Using high-resolution mass spectrometry-based quantitative proteomics of the bovine whey proteins, we identified a total of 1459 and 1358 proteins in biological replicates, out of which 220 and 157 proteins were differentially expressed between normal and infected samples. A total of...Continue Reading

References

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Citations

Apr 11, 2021·Journal of Proteomics·André M AlmeidaMaya Zachut

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Datasets Mentioned

BETA
PXD014922
33

Methods Mentioned

BETA
electrophoresis
nuclear translocation

Software Mentioned

ClueGo
BiNGO
Search Tool for the Retrieval of Interacting Genes / Proteins ...
Protein Analysis Through Evolutionary Relationships ( PANTHER )
Protein Scape
Cluster
- viz
the
Mascot
CODE

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