Tannin interactions with a full-length human salivary proline-rich protein display a stronger affinity than with single proline-rich repeats

FEBS Letters
Adrian J CharltonMike P Williamson

Abstract

The protein IB5 has been purified from human parotid saliva. This protein contains several repeats of a short proline-rich sequence. Dissociation constants have been measured at several discrete binding sites using 1H-NMR for the hydrolysable tannins (polyphenols) beta-1,3,6-tri-O-galloyl-D-glucopyranose, beta-1,2,4,6-tetra-O-galloyl-D-glucopyranose and beta-1,2,3,4,6-penta-O-galloyl-D-glucopyranose and the condensed proanthocyanidin (--)-epicatechin. The dissociation constants for trigalloyl glucose and pentagalloyl glucose were 15 X 10(-5) and 1.7 X 10(-5) M, respectively, which are 115 and 1660 times stronger than those previously measured under the same conditions for a single repeat of a mouse salivary proline-rich protein. The increase in affinity is ascribed to intramolecular secondary interactions, which are strengthened by the rigidity of the interacting molecules.

References

Jan 1, 1977·Archives of Oral Biology·M Levine, P J Keller
Jan 1, 1979·Archives of Oral Biology·D L Kauffman, P J Keller
Feb 1, 1987·Journal of Dental Research·A Bennick
Oct 1, 1968·Archives of Oral Biology·J C Steiner, P J Keller
Jan 1, 1984·Methods in Enzymology·C R MerrilM L Van Keuren
Feb 1, 1994·European Journal of Biochemistry·N J Murray, M P Williamson
Jan 15, 1994·The Biochemical Journal·M P Williamson

❮ Previous
Next ❯

Citations

Oct 20, 2009·Analytical and Bioanalytical Chemistry·Francis CanonPascale Sarni-Manchado
Jul 29, 2010·Analytical and Bioanalytical Chemistry·Francis CanonPascale Sarni-Manchado
Feb 1, 2011·Plant Molecular Biology·Ryann M MuirAbhaya M Dandekar
Sep 29, 2011·Journal of Agricultural and Food Chemistry·Rui GonçalvesVictor De Freitas
Sep 1, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Rui GonçalvesVictor de Freitas
Jan 5, 2012·Critical Reviews in Food Science and Nutrition·C Le Bourvellec, C M G C Renard
Oct 22, 2008·Tissue Engineering. Part a·Mary E TedderDan T Simionescu
Mar 4, 2009·Tissue Engineering. Part a·Ting-Hsien ChuangDan T Simionescu
Dec 23, 2011·International Journal of Dentistry·Martin Levine
Jul 26, 2012·Oxidative Medicine and Cellular Longevity·Abdelkader BasliTristan Richard
Sep 6, 2003·Biological Chemistry·Jürgen PolsterFeucht Walter
Mar 4, 2010·Archives of Oral Biology·Erika SillettiMonique H Vingerhoeds
Aug 19, 2007·Archives of Oral Biology·Raymond G SchipperMonique H Vingerhoeds
Sep 22, 2010·Journal of Peptide Science : an Official Publication of the European Peptide Society·Elżbieta Kamysz, Emilia Sikorska
Nov 25, 2004·European Journal of Oral Sciences·Andrew JoinerThomas Arnebrant
Apr 17, 2015·Journal of Agricultural and Food Chemistry·Jacqui M McRaePaul A Smith
Jan 16, 2014·Annual Review of Food Science and Technology·L Federico Casassa, James F Harbertson
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Anders Bennick
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R Matsuo
Jun 8, 2017·Journal of Agricultural and Food Chemistry·Mafalda Santos SilvaSusana Soares
Aug 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Peter Tompa
Mar 15, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jacqui M McRae, James A Kennedy
Apr 19, 2008·Organic & Biomolecular Chemistry·A K Croft, M K Foley
Aug 21, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Y ChungC S Yang
Jul 8, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Olivier CalaIsabelle Pianet
Jan 13, 2011·Angewandte Chemie·Stéphane QuideauLaurent Pouységu
Mar 1, 2005·The Journal of Nutrition·Hee-Seon Kim, Dennis D Miller
Oct 10, 2018·Annals of Biomedical Engineering·Sourav S PatnaikEnder A Finol
Mar 23, 2018·Critical Reviews in Biochemistry and Molecular Biology·Federica IavaroneTiziana Cabras
Aug 5, 2017·Nutrition & Metabolism·Nicole M DelimontBrian L Lindshield
Dec 31, 2016·Food Science and Biotechnology·Min Young KimHeon Sang Jeong
Sep 5, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Isabelle PianetMichel Laguerre

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.