Tanshinone IIA, an isolated compound from Salvia miltiorrhiza Bunge, induces apoptosis in HeLa cells through mitotic arrest

Life Sciences
Lingli ZhouDonald C Chang

Abstract

Tanshinone IIA (Tan IIA) is a compound isolated from Salvia miltiorrhiza Bunge (Danshen). The aim of this study is to investigate the mechanisms of its anti-cancer effect. To clearly delineate the cell cycle-dependent effects of Tan IIA, we used either synchronized cells or single living cell analysis to conduct our studies. Subcellular fractionation, Western blot analysis, immuno-fluorescence staining and FACS analysis were also employed in our study. We found that Tan IIA could arrest cancer cells in mitosis by disrupting the mitotic spindle and subsequently triggered cells to enter apoptosis through the mitochondria-dependent apoptotic pathway. Thus, Tan IIA could selectively kill mitotic cells over interphase cells. In comparison with other existing anti-cancer drugs that cause mitotic arrest by interfering with the microtubule structure (such as vincristine or taxol), Tan IIA destroyed only the mitotic spindle during the M phase but not the microtubule structure in interphase cells. Furthermore, Tan IIA could trigger the mitotic arrested cells to enter apoptosis faster than vincristine or taxol. Since Tan IIA can selectively induce cancer cells to enter apoptosis through mitotic arrest, it has the potential to be developed...Continue Reading

References

Feb 27, 1999·Current Opinion in Cell Biology·S Karki, E L Holzbaur
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Sep 29, 1999·Cancer Chemotherapy and Pharmacology·L G WangD R Budman
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Mar 21, 2001·Cell·S J RiedlG S Salvesen
Dec 13, 2002·Cell Death and Differentiation·M CastedoG Kroemer
Sep 17, 2003·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhongzhi TangLibo Fu
Nov 5, 2003·Advanced Drug Delivery Reviews·John W Murray, Allan W Wolkoff
Nov 25, 2003·Oncogene·Alexei DegterevJunying Yuan
Dec 10, 2003·Oncogene·Kapil N Bhalla
Mar 5, 2004·Biochimica Et Biophysica Acta·Juanita C SharpeRichard J Youle
Jul 9, 2004·World Journal of Gastroenterology : WJG·Shu-Lan YuanJie Zhang
Jan 13, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jennifer R Levy, Erika L F Holzbaur
Oct 16, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lin AoJia Cao
Jan 29, 2008·Current Cancer Drug Targets·Mary Ann Jordan, Kathy Kamath

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Citations

Sep 13, 2012·Chemical Reviews·Yi-Bing WuBin Cong
Jan 18, 2012·BMC Complementary and Alternative Medicine·Chang LiuMo Yang
Dec 4, 2012·International Journal of Molecular Sciences·Yong ZhangJunxuan Lü
Mar 25, 2010·Immunopharmacology and Immunotoxicology·Zahra AmirghofranFatemeh Kalalinia
Jun 25, 2013·Expert Opinion on Investigational Drugs·Su-Juan WangLu-Ping Qin
Jul 20, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jian FangPei-Qing Liu
Oct 15, 2013·Medicinal Research Reviews·Xiuping ChenYitao Wang
May 16, 2014·BioMed Research International·Jiang-Feng WangWei-Min Mao
Jul 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Li QiaoQingcong Wei
May 21, 2019·Chemical Biology & Drug Design·Defeng XuHuan Zhou
Feb 19, 2021·Frontiers in Pharmacology·Zhong-Ying FangLei Fang

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