Tanshinone IIA attenuates cardiac microvascular ischemia-reperfusion injury via regulating the SIRT1-PGC1α-mitochondrial apoptosis pathway

Cell Stress & Chaperones
Jiankai ZhongYunzhao Hu

Abstract

Cardiac microvascular ischemia-reperfusion (IR) injury has been a neglected topic in recent decades. In the current study, we investigated the mechanism underlying microvascular IR injury, with a focus on mitochondrial homeostasis. We also explored the protective role of tanshinone IIA (Tan IIA) in microvascular protection in the context of IR injury. Through animal studies and cell experiments, we demonstrated that IR injury mediated microvascular wall destruction, lumen stenosis, perfusion defects, and cardiac microvascular endothelial cell (CMEC) apoptosis via inducing mitochondrial damage. In contrast, Tan IIA administration had the ability to sustain CMEC viability and microvascular homeostasis, finally attenuating microvascular IR injury. Function studies have confirmed that the SIRT1/PGC1α pathway is responsible for the microvascular protection from the Tan IIA treatment. SIRT1 activation by Tan IIA sustained the mitochondrial potential, alleviated the mitochondrial pro-apoptotic factor leakage, reduced the mPTP opening, and blocked mitochondrial apoptosis, providing a survival advantage for CMECs and preserving microvascular structure and function. By comparison, inhibiting SIRT1 abrogated the beneficial effects of Tan ...Continue Reading

References

Jan 24, 2017·International Journal of Radiation Biology·Shunying HuYundai Chen
Sep 7, 2017·Journal of Pineal Research·Wenkai RenYulong Yin
Oct 6, 2017·Redox Biology·Mini ChandraManikandan Panchatcharam
Oct 29, 2017·Redox Biology·C CheignonF Collin
Nov 8, 2017·Angiogenesis·Sanaullah SajibConstantinos M Mikelis
Nov 18, 2017·Journal of Pineal Research·Lauren A E ErlandPraveen K Saxena
Dec 17, 2017·Journal of Pineal Research·Roohi JeelaniHusam M Abu-Soud
Feb 14, 2018·Journal of Pineal Research·Romesh G AbeysuriyaSvetlana Postnova
Jun 15, 2018·Cell Death and Differentiation·Mart BittremieuxGeert Bultynck
Jul 1, 2018·Cell Death and Differentiation·Donna DentonSharad Kumar
Jul 22, 2018·Cell Death and Differentiation·Anna L AaltoAnnika Meinander
Aug 8, 2018·Basic Research in Cardiology·Jing LiAndrew N Redington

❮ Previous
Next ❯

Citations

Oct 12, 2020·Pharmacology & Therapeutics·Chunhui ZhaoLuqi Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis