Tanshinone IIA enhances the effects of TRAIL by downregulating survivin in human ovarian carcinoma cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Jyun-Yi LinTsing-Fen Ho

Abstract

Tanshinone IIA (TIIA), a diterpene quinone from the medicinal plant Salvia miltiorrhiza Bunge (Lamiaceae) was shown to possess apoptotic and TRAIL-sensitizing effects. Still, the molecular mechanisms whereby TIIA induces apoptosis remain largely unknown. The role of survivin, an inhibitor of apoptosis protein, in TIIA-induced apoptosis has never been addressed before and hence was the primary goal of this study. In this study, we explored the anticancer effect of TIIA in TOV-21G, SKOV3, and OVCAR3 ovarian carcinoma cells. Cytotoxicity was determined by MTS assay. Real-time RT-PCR and Western blotting were used to assess the mRNA and protein expression of related signaling proteins. Our results illustrated that TIIA's cytotoxic effect was caused by apoptosis with the involvement of caspases activity. Moreover, TIIA downregulated survivin in a concentration-dependent manner without affecting the expression of Bcl-2, Bcl-xL, and Bax. TIIA-induced survivin downregulation is regulated by both transcriptional processes and proteasomal degradation. Using TOV-21G cells as our cellular model, we demonstrated that TIIA-induced survivin downregulation requires p38 MAPK activation. Importantly, genetic overexpression of survivin rendered c...Continue Reading

References

Jun 17, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Cynthia CohenRobert Santoianni
Mar 26, 2005·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Caroline M M Van GeelenSteven de Jong
Feb 13, 2007·Cancer Cell·Ignacio DoladoAngel R Nebreda
Sep 21, 2007·Toxicology and Applied Pharmacology·Tsing-Fen HoChia-Che Chang
Jan 10, 2009·Toxicology and Applied Pharmacology·Tsing-Fen HoChia-Che Chang
Jul 24, 2010·The American Journal of Pathology·Ernst Lengyel
Aug 17, 2010·Chemico-biological Interactions·Huei-Fang LiuJui-I Chao
Apr 8, 2011·Molecular Cancer·Ronan J KellyJohn C Morris
Feb 15, 2012·Journal of Experimental & Clinical Cancer Research : CR·Ayako KimTakayuki Enomoto
May 25, 2012·Bioorganic & Medicinal Chemistry Letters·Byung Jun RyuSeong Hwan Kim
Jan 12, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Susana Banerjee, Stanley B Kaye
May 11, 2013·Journal of Bioscience and Bioengineering·Chia-Che ChangTsing-Fen Ho
Aug 21, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Bernd Groner, Astrid Weiss
Oct 9, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shunzeng LvHong-Shuo Sun
Feb 25, 2014·Cancer Letters·Kalliopi Ch AthanasoulaMarina Mantzourani
Jun 21, 2014·Cell Death and Differentiation·J LemkeH Walczak

❮ Previous
Next ❯

Citations

Dec 2, 2015·BioMedicine·Tsing-Fen Ho, Chia-Che Chang
Apr 22, 2016·Cancer Chemotherapy and Pharmacology·Xiaoping JingLi He
Jun 27, 2018·The Journal of Pharmacy and Pharmacology·Nan LiSuqin Chen
Sep 15, 2018·Experimental and Therapeutic Medicine·Yongjun ZhangTiansheng Cao
Apr 4, 2017·Phytotherapy Research : PTR·Muhammad Torequl Islam
Feb 19, 2021·Frontiers in Pharmacology·Zhong-Ying FangLei Fang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.