Tanshinone IIA Prevents Rat Basilar Artery Smooth Muscle Cells Proliferation by Inactivation of PDK1 During the Development of Hypertension

Journal of Cardiovascular Pharmacology and Therapeutics
Zhi-Liang YuJian-Ming Jiang

Abstract

Basilar vascular smooth muscle cells (BASMCs) hyperplasia is a prominent feature of cerebrovascular remodeling and stroke during the development of hypertension. Tanshinone IIA (Tan) has been reported to exhibit a protective effect against the pathological features of hypertension. Previous studies have shown that phosphoinostitide-3 kinase (PI3K)/3'-phosphoinostitide dependent kinase (PDK1)/AKT pathway is involved in the regulation of proliferation of various cell types. Therefore, there may be a crosstalk between Tan antihypertension processes and PI3K/PDK1/AKT proliferative effect in BASMCs. To test this hypothesis, we used a 2-kidney, 2-clip hypertension model to examine the effect of Tan on PI3K/PDK1/AKT pathway by cellular, molecular, and biochemical approaches. Our results revealed that the abundance of PDK1 in plasma was paralleled with an increase in blood pressure and the cross-sectional area of basilar artery in hypertensive rats. Tan decreased blood pressure and hypertension-induced PDK1 phosphorylation but produced no effect on the phosphorylation of PI3K. Moreover, Tan attenuated endothelin 1 induced the activation of PDK1/AKT pathway in rat BASMCs. Tan could inhibit cell cycle transition by regulating the express...Continue Reading

References

Feb 12, 1998·The Journal of Biological Chemistry·M AndjelkovićB A Hemmings
Aug 26, 1998·Stroke; a Journal of Cerebral Circulation·J ZengR Huang
Sep 7, 2000·Circulation·M R WardC Borst
Jun 20, 2001·American Journal of Hypertension·E L Schiffrin
Nov 1, 2001·Annual Review of Cell and Developmental Biology·R KatsoM D Waterfield
Jun 24, 2004·The Journal of Biological Chemistry·Charles C King, Alexandra C Newton
Nov 18, 2005·Journal of Clinical Pharmacology·Limin ZhouMoses Sing Sum Chow
Dec 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·David D KimWalter N Durán
Oct 20, 2009·Journal of Molecular and Cellular Cardiology·Catherine A LemariéStéphanie Lehoux
Oct 15, 2013·The American Journal of Chinese Medicine·Christian StumpfChristoph D Garlichs
Jun 24, 2014·European Journal of Pharmacology·Suzanne A Nasser, Mahmoud M El-Mas
Jul 13, 2014·European Journal of Pharmacology·Rocío Alcántara-HernándezJ Adolfo García-Sáinz

❮ Previous
Next ❯

Citations

Apr 27, 2018·Acta Pharmacologica Sinica·Zhuo-Ming LiPei-Qing Liu
Nov 26, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhibei LiXiao Ma

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
electrophoresis
flow cytometry
transfection

Software Mentioned

SPSS
ImageJ

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.