Tanshinone IIA promotes the apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis by up-regulating lncRNA GAS5

Bioscience Reports
Guoqing LiDan Liu

Abstract

Rheumatoid arthritis (RA) is a common chronic autoimmune joint disease characteristic of elevated proliferation and infiltration of fibroblast-like synoviocytes (FLS). Here, we aimed to explore the mechanisms of the Tanshinone IIA (Tan IIA)-induced apoptosis of FLS from patients with RA (termed RAFLS). Cell Counting Kit-8 (CCK-8) assay and Annexin V staining revealed that RAFLS viability decreased and apoptosis increased after Tan IIA treatment. Long non-coding RNA (lncRNA) GAS5 expression was significantly decreased in the synovial tissues and RAFLS, while Tan IIA treatment resulted in an up-regulation of GAS5. Consistently, knockdown of GAS5 using siRNA inhibited RAFLS apoptosis. Mechanistically, GAS5 knockdown down-regulated the expression of cleaved caspase-3 and caspase-9 in the RAFLS cells and activated the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway. These data indicate that Tan IIA promotes RAFLS apoptosis by up-regulating lncRNA GAS5, with enhanced expression of cleaved caspase-3/caspase-9 and inhibited PI3K/AKT signaling.

References

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Citations

Mar 19, 2020·Chinese Medical Journal·Min-Xi Lao, Han-Shi Xu
Oct 3, 2020·Bioengineering & Translational Medicine·Louis J BornSteven M Jay
Aug 28, 2019·Journal of Cellular Biochemistry·Ruhai YiYueqing Su
Dec 16, 2020·Journal of Clinical Laboratory Analysis·Weiwei LiuXiaodan Mo
Feb 28, 2021·Genes & Genomics·Sabrina Ka Man Tam, Danny Chi Yeu Leung
Feb 22, 2021·Molecular and Cellular Biochemistry·Tao PengYankai Jiang
Aug 4, 2020·Journal of Translational Autoimmunity·Yaoyao Zou, Hanshi Xu
Apr 19, 2019·Journal of Translational Internal Medicine·Junyu LiangJin Lin
Feb 24, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Miao-Juan ShiHui Zhang

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Methods Mentioned

BETA
transfection
PCR
Protein Assay
Assay
FACS

Software Mentioned

Quantity One Image
SAS
CellQuest

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