TAO1, a representative of the molybdenum cofactor containing hydroxylases from tomato.

The Journal of Biological Chemistry
N OriR Fluhr

Abstract

Aldehyde oxidase and xanthine dehydrogenase are a group of ubiquitous hydroxylases, containing a molybdenum cofactor (MoCo) and two iron-sulfur groups. Plant aldehyde oxidase and xanthine dehydrogenase activities are involved in nitrogen metabolism and hormone biosynthesis, and their corresponding genes have not yet been isolated. Here we describe a new gene from tomato, which shows the characteristics of a MoCo containing hydroxylase. It shares sequence homology with xanthine dehydrogenases and aldehyde oxidases from various organisms, and similarly contains binding sites for two iron-sulfur centers and a molybdenum-binding region. However, it does not contain the xanthine dehydrogenase conserved sequences thought to be involved in NAD binding and in substrate specificity, and is likely to encode an aldehyde oxidase-type activity. This gene was designated tomato aldehyde oxidase 1 (TAO1). TAO1 belongs to a multigene family, whose members are shown to map to clusters on chromosomes 1 and 11. MoCo hydroxylase activity is shown to be recognized by antibodies raised against recombinant TAO1 polypeptides. Immunoblots reveal that TAO1 cross-reacting material is ubiquitously expressed in various organisms, and in plants it is mostly ...Continue Reading

References

Jun 1, 1972·Archives of Biochemistry and Biophysics·T A KrenitskyG H Hitchings
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Feb 10, 1995·The Journal of Biological Chemistry·A SatoT Nishino
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R M WrightJ E Repine
Dec 29, 1995·The Journal of Biological Chemistry·M L CalziE Garattini

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Citations

Oct 16, 2012·Plant Molecular Biology·Maryam ZarepourFlorian Bittner
Apr 25, 2000·Plant Science : an International Journal of Experimental Plant Biology· Katalin Barabás N Herman Lips S
Sep 6, 2000·The Plant Journal : for Cell and Molecular Biology·M SeoT Koshiba
Aug 8, 2002·The Plant Journal : for Cell and Molecular Biology·Moshe SagiRobert Fluhr
Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SeoT Koshiba
Nov 17, 2007·Plant Physiology and Biochemistry : PPB·Edyta Zdunek-Zastocka
Sep 28, 2000·Journal of Experimental Botany·I B TaylorA J Thompson
Aug 5, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·L S Barrero, S D Tanksley
May 5, 2006·The Journal of Biological Chemistry·Mineko TeraoEnrico Garattini

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