Tapered-slit membrane filters for high-throughput viable circulating tumor cell isolation

Biomedical Microdevices
Yoon-Tae KangYoung-Ho Cho

Abstract

This paper presents tapered-slit membrane filters for high-throughput viable circulating tumor cell (CTC) isolation. The membrane filter with a 2D array of vertical tapered slits with a gap that is wide at the entrance and gradually decreases with depth, provide minimal cell stress and reduce 82.14% of the stress generated in conventional straight-hole filters. We designed two types of tapered-slit filters, Filters 6 and 8, respectively, containing the tapered slits with outlet widths of 6 μm and 8 μm at a slit density of 34,445/cm(2) on the membrane. We fabricated the vertical slits with a tapered angle of 2 ° on a SU8 membrane by adjusting the UV expose dose and the air gap between the membrane and the photomask during lithography. In the experimental study, the proposed tapered-slit filter captured 89.87% and 82.44% of the cancer cells spiked in phosphate buffered saline (PBS) and diluted blood (blood: PBS = 1:4), respectively, at a sample flow rate of 5 ml per hour, which is 33.3 times faster than previous lateral tapered-slit filters. We further verified the capability to culture on chip after capturing: 72.33% of cells among the captured cells still remained viable after a 5-day culture. The proposed tapered-slit membrane...Continue Reading

References

Jul 1, 1970·The American Journal of Physiology·S ChienM I Gregersen
Aug 20, 2004·The New England Journal of Medicine·Massimo CristofanilliDaniel F Hayes
Jul 22, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daniel F HayesLeon W W M Terstappen
Oct 26, 2007·Lab on a Chip·Sungyoung ChoiJe-Kyun Park
Mar 24, 2009·Electrophoresis·Peter R C GascoyneFrederick F Becker
Jun 30, 2010·Analytical Chemistry·Masahito HosokawaTadashi Matsunaga
Oct 28, 2010·Biomedical Microdevices·Siyang ZhengYu-Chong Tai
May 9, 2012·Lab on a Chip·Colette A BichselMatthias P Lutolf

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Citations

Jul 11, 2018·Biotechnology and Bioengineering·Sultan KhetaniAmir Sanati Nezhad
Nov 5, 2017·Oncotarget·Dong Hoon SuhYoung-Ho Cho
Jan 14, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yupeng Hong, Qi Zhang
Nov 28, 2018·Micromachines·Yaoping LiuWei Wang
Aug 17, 2020·Biomedical Microdevices·Lujing SunLianqing Liu

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