PMID: 395103Dec 1, 1979Paper

Tapping culture--an improved method for cell suspension culture

In Vitro
H KatsutaH Ito


A new culture vessel was designed for cell suspension culture. A silicone-covered magnet bar fixed by one end to the side wall of the bottle was held horizontally a short distance from the bottom. A standard type magnetic stirrer was used. In contrast to the conventional horizontal movement of "stirring" in cultures the bar moves vertically with a "tapping" motion. This improvement resulted in less cell injury, higher rate of cell proliferation and formation of fewer bubbles than in the conventional type. Nine cell types were simultaneously cultivated in tapping, stirring and stationary culture. All cell types proliferated more luxuriously in tapping cultures then in stirring cultures. Serial cultivation of cells in tapping cultures was also successful.


Jan 1, 1969·Zeitschrift für Krebsforschung·O CostăchelE Badea
Sep 1, 1966·Experimental Cell Research·P F Rakowski, G L Tritsch
Feb 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·D J MERCHANT, R H KAHN
Nov 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·B BJORKLUNDJ E PAULSSON
Feb 1, 1964·Experimental Cell Research·S J PIRT, D S CALLOW
Nov 17, 1954·Annals of the New York Academy of Sciences·W R EARLEE L SCHILLING

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