Taqman-MGB nanoPCR for Highly Specific Detection of Single-Base Mutations.

International Journal of Nanomedicine
Zhenrui XueChunyan Yao

Abstract

Detection of single-base mutations is important for real-time monitoring of tumor progression, therapeutic effects, and drug resistance. However, the specific detection of single-base mutations from excessive wild-type background sequences with routine PCR technology remains challenging. Our objective is to develop a simple and highly specific qPCR-based single-base mutation detection method. Using EGRF T790M as a model, gold nanoparticles at different concentrations were separately added into the Taqman-MGB qPCR system to test specificity improvement, leading to the development of the optimal Taqman-MGB nanoPCR system. Then, these optimal conditions were used to test the range of improvement in the specificity of mutant-type and wild-type templates and the detection limit of mutation abundances in a spiked sample. The Taqman-MGB nanoPCR was established based on the traditional qPCR, with significantly suppressed background noise and improved specificity for single-base mutation detection. With EGFR T790M as a template, we demonstrated that our Taqman-MGB nanoPCR system could improve specificity across a wide concentration range from 10-9 μM to 10 μM and detect as low as 0.95% mutation abundance in spiked samples, which is lowe...Continue Reading

References

Jun 9, 2005·Angewandte Chemie·Haikuo LiJun Hu
Nov 30, 2005·Nucleic Acids Research·Min LiHsiao-Sheng Liu
Sep 6, 2011·Nature Nanotechnology·Peng ChenYongyong Shi
Aug 22, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Dun PanChunhai Fan
Jun 6, 2013·ACS Applied Materials & Interfaces·Xinhui Lou, Ying Zhang
Nov 26, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Audrey ValléeMarc G Denis
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jonathan R HartPeter K Vogt
Jun 27, 2015·ACS Applied Materials & Interfaces·Ji Young ParkTae Jung Park
Sep 8, 2016·ACS Applied Materials & Interfaces·Aijun LiXueyan Cao
Dec 3, 2016·Ecancermedicalscience·Rosa A van AmerongenWim H van Harten
Jan 29, 2017·Expert Review of Molecular Diagnostics·Umberto MalapelleCarlos Camps
Feb 12, 2017·Cell·Alexander M AravanisRichard D Klausner
Jun 19, 2017·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Xuefei LiFred R Hirsch
Nov 11, 2019·Clinical Chemistry·Stephen J Salipante, Keith R Jerome
Jan 29, 2020·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D PlanchardS Peters
Feb 14, 2020·Nature·Jennifer A Doudna
Mar 23, 2020·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Inger Johanne Zwicky EideOdd Terje Brustugun

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