TaqMan qPCR for Quantification of Clonostachys rosea Used as a Biological Control Agent Against Fusarium graminearum

Frontiers in Microbiology
Alejandro GimenoSusanne Vogelgsang

Abstract

Clonostachys rosea is a biological control agent against Fusarium graminearum in small grain cereals and maize. Infections with F. graminearum do not only reduce the yield but, due to the production of mycotoxins, also affect the entire value chain of food and feed. In addition, production of other secondary metabolites such as hydrophobins, also known as gushing inducers, may cause quality challenges for the malting and brewing industry. Sustainable disease control strategies using C. rosea are treatment of infected residues of the previous crop, direct treatment of the actual cereal crop or post-harvest treatment during malting processes. Follow-up of growth and survival of biocontrol organisms during these different stages is of crucial importance. In the current study, we developed a quantitative real-time PCR detection method that amends the currently available culture-dependent techniques by using TaqMan chemistry with a highly specific primer and probe set, targeting the actin gene. We established a sensitive assay that detects the biological control agent down to 100 genome copies per reaction, with PCR efficiencies between 90 and 100%. The specificity of the assay was confirmed against a panel of 30 fungal and 3 bacter...Continue Reading

References

Aug 8, 2007·Journal of Industrial Microbiology & Biotechnology·Arja LaitilaAnnika Wilhelmson
Jun 23, 2009·Journal of Microbiological Methods·Salome SchneiderFranco Widmer
Nov 1, 2004·Molecular Plant Pathology·Rubella S Goswami, H Corby Kistler
Jul 23, 2011·Journal of Basic Microbiology·Tuija SarlinTiina Nakari-Setälä
Nov 10, 2011·Phytopathology·Guillaume J BilodeauFrank N Martin
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Jan 13, 2015·Genome Biology and Evolution·Magnus KarlssonDan Funck Jensen
Jun 25, 2016·Genome Announcements·Shuai LiuXiaojiang Hao

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Datasets Mentioned

BETA
MN052804
MN052805

Methods Mentioned

BETA
PCA
PCR
electrophoresis

Software Mentioned

BLASTtn
BLASTn
SPSS Statistics
Geneious
VTTact
Primer
Excel
BLAST
ClustalO
Lightcycler 480

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