TaqMan quantitative real-time PCR for detecting Avipoxvirus DNA in various sample types from hummingbirds

PloS One
Hanna Eugene BaekLisa A Tell

Abstract

Avian pox is a viral disease documented in a wide range of bird species. Disease-related detrimental effects can cause dyspnea and dysphagia, and birds with high metabolic requirements, such as hummingbirds, are thus especially vulnerable to the pathogen. Hummingbirds have a strong presence in California, especially in urban environments. However, little is understood regarding the impact of pox virus on hummingbird populations. Currently, diagnosing a pox infection relies on obtaining a tissue biopsy, which poses significant risks to birds and challenges in the field. Understanding the ecology of hummingbird pox viral infections could be advanced by a minimally invasive ante-mortem diagnostic method. Our aim was to address whether pox infections can be diagnosed using integumentary system samples besides tissue biopsies. To meet this goal, we tested multiple integumentary sample types using a quantitative real-time PCR assay. A secondary study goal was to determine which sample types (ranging from minimally to highly invasive sampling) were optimal for identifying infected birds. Pox-like lesion tissue, pectoral muscle, feathers, toenail clippings, blood, and swabs (both pox-like lesion tissue and non pox-like lesion tissue) w...Continue Reading

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Citations

Oct 14, 2021·The Journal of Parasitology·A N GalvinR N M Sehgal

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Datasets Mentioned

BETA
JX418296.1
MT332851

Methods Mentioned

BETA
biopsy
biopsies
PCR
electrophoresis
dissection

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
Primer Express
Geneious®
BLAST

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