Target-assembled exciplexes based on Scorpion oligonucleotides

Bioscience Reports
Abdul GbajK T Douglas

Abstract

Scorpion probes, specific DNA probe sequences maintained in a hairpin-loop, can be modified to carry the components of an exciplex for use as a novel fluorescence-based method for specific detection of DNA. The exciplex partners (5'-pyrenyl and 3'-naphthalenyl) were attached to oligonucleotides via phosphoramidate links to terminal phosphate groups. Hybridization of the probe to a complementary target in a buffer containing trifluoroethanol produced an obvious fluorescence change from blue (pyrene locally excited state emission) to green (exciplex emission).

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Oct 17, 2007·Journal of Biomolecular Structure & Dynamics·Lindsey WalshKenneth T Douglas

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Citations

Nov 30, 2010·Biochimica Et Biophysica Acta·Elena V BichenkovaKenneth T Douglas
Jan 14, 2010·Angewandte Chemie·Robert HänerVladimir L Malinovskii

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