Target gene approaches: Gene expression in Daphnia magna exposed to predator-borne kairomones or to microcystin-producing and microcystin-free Microcystis aeruginosa.

BMC Genomics
Anke SchwarzenbergerEric von Elert

Abstract

Two major biological stressors of freshwater zooplankton of the genus Daphnia are predation and fluctuations in food quality. Here we use kairomones released from a planktivorous fish (Leucaspius delineatus) and from an invertebrate predator (larvae of Chaoborus flavicans) to simulate predation pressure; a microcystin-producing culture of the cyanobacterium Microcystis aeruginosa and a microcystin-deficient mutant are used to investigate effects of low food quality. Real-time quantitative polymerase chain reaction (QPCR) allows quantification of the impact of biotic stressors on differential gene activity. The draft genome sequence for Daphnia pulex facilitates the use of candidate genes by precisely identifying orthologs to functionally characterized genes in other model species. This information is obtained by constructing phylogenetic trees of candidate genes with the knowledge that the Daphnia genome is composed of many expanded gene families. We evaluated seven candidate reference genes for QPCR in Daphnia magna after exposure to kairomones. As a robust approach, a combination normalisation factor (NF) was calculated based on the geometric mean of three of these seven reference genes: glyceraldehyde-3-phosphate dehydrogena...Continue Reading

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Citations

Aug 21, 2013·Aquatic Toxicology·Shruti Pavagadhi, Rajasekhar Balasubramanian
Aug 30, 2011·Integrative and Comparative Biology·Ianina AltshulerMelania E Cristescu
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Mar 22, 2014·Proceedings. Biological Sciences·Christoph Effertz, Eric von Elert
Mar 20, 2014·Aquatic Toxicology·Thomas Sadler, Eric von Elert
Sep 10, 2014·BMC Genomics·Anke SchwarzenbergerEric von Elert
Jan 17, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ramona DöllingBettina Zeis
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Methods Mentioned

BETA
PCR
electrophoreses

Software Mentioned

BLASTp
wFleabase
MEGA
BLASTn
qBase
geNorm
Excel
BioEdit

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